Characterization of enterohemorrhagic Escherichia coli O157:H7 00B015: a Shiga toxin producing but virulence-attenuated isolate

被引：0

作者：

Wang, Haiguang ^{[1
]}

Gu, Jiang ^{[1
]}

Yu, Shu ^{[1
]}

Zhang, Weijun ^{[1
]}

Zhu, Yefei ^{[2
]}

Zou, Quanming ^{[1
]}

Zhu, Fengcai ^{[2
]}

Mao, Xuhu ^{[1
]}

机构：

[1] Third Mil Med Univ, Dept Clin Microbiol & Immunol, Coll Med Lab Sci, Chongqing 400038, Peoples R China

[2] Jiangsu Prov Ctr Dis Control & Prevent, Nanjing 210009, Peoples R China

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2010年 / 56卷 / 08期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

enterohemorrhagic Escherichia coli; Shiga toxin; actin polymerization; ACTIN POLYMERIZATION; HEMORRHAGIC COLITIS; O157-H7; TIR; INTIMIN; INFECTIONS; PROTECTION; EFFECTOR; ESPF(U); VACCINE;

D O I：

10.1139/W10-049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enterohemorrhagic Escherichia coli (EHEC) causes a wide range of systematic diseases in human and animals in 2 main ways: (1) production of Shiga toxin (Stx) and (2) induction of actin polymerization characterized by attaching and effacing (A/E) lesions. Stx is commonly targeted in the development of drugs and vaccines to control EHEC infection for its indispensible contribution to EHEC pathogenisis. In this study, we isolated a Stx-producing EHEC O157:H7 isolate 00B015 and found that its ability to induce actin polymerization was impaired. In addition, it reduces pathogenicity and decreases mortality in mice. Our results report a Stx-producing but virulence-attenuated EHEC isolate 00B015 and suggest that the formation of actin polymerization may help Stx-induced pathogenesis and have a more important contribution in EHEC infections.

引用

页码：651 / 656

页数：6

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