1.8-megabases fine physical map encompassing IFNAR and AML1 loci on human chromosome 21q22.1

被引：5

作者：

Eki, T

Abe, M

Furuya, K

Fujishima, N

Kishida, H

Shiratori, A

Yokoyama, K

LePaslier, D

Cohen, D

Murakami, Y

机构：

[1] INST PHYS & CHEM RES, TSUKUBA LIFE SCI CTR, GENE BANK, DNA BANK, TSUKUBA, IBARAKI 305, JAPAN

[2] CTR ETUD POLYMORPHISME HUMAIN, FDN JEAN DAUSSET, F-75010 PARIS, FRANCE

来源：

DNA SEQUENCE | 1996年 / 6卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

CpG island; human chromosome 21; physical map; rare cutting enzyme; sequence-tagged site; yeast artificial chromosome;

D O I：

10.3109/10425179609010196

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A long-range restriction map of the 1.8-megabases (mb) region encompassing the area between the interferon-alpha receptor and the acute myelogenous leukemia loci on human chromosome 21q22.1 was constructed after analysis of both the contiguous yeast artificial chromosome (YAC) clones and genomic DNA. Analysis of pulsed-field gel electrophoresis of lymphoblastoid DNA digested with three rare-cutting enzymes, Not I, Mlu I, and Nru I, revealed the positions of 17 markers on each restriction map. The 1.8-mb YAC contig that covers this region was obtained through YAC walking mediated by sequence-tagged sites (STSs), with 29 STSs including 12 newly generated YAC end-specific STSs. The consensus restriction map from 15 overlapping YACs and the positioning of the STS markers on each clone allowed 24 markers including 4 Not I-linking STSs to be ordered and mapped physically. Comparison of the maps revealed that the proximal region contains more unmethylated CpG islands than the distal region, which suggests that many expressed genes are in the proximal region. This fine consensus physical map will be informative and useful for construction of contigs of cosmid, P1, or BAC clones for further large-scale sequencing in this gene-rich region.

引用

页码：95 / 108

页数：14

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