Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R

被引：2

作者：

Bourquain, Daniel ^{[1
]}

Schrick, Livia ^{[1
]}

Tischer, Bernd Karsten ^{[2
]}

Osterrieder, Klaus ^{[2
,3
]}

Schaade, Lars ^{[1
]}

Nitsche, Andreas ^{[1
]}

机构：

[1] Robert Koch Inst, Ctr Biol Threats & Special Pathogens 1, Seestr 10, D-13353 Berlin, Germany

[2] Free Univ Berlin, Inst Virol, Dept Vet Med, Robert von Ostertag Str 7-13, D-14163 Berlin, Germany

[3] City Univ Hong Kong, Jockey Club Coll Vet Med & Life Sci, Dept Infect Dis & Publ Hlth, Kowloon, Hong Kong, Peoples R China

来源：

VIROLOGY JOURNAL | 2021年 / 18卷 / 01期

关键词：

Orthopoxvirus; Cowpox virus; Host range; p28; RING finger; RING FINGER PROTEIN; UBIQUITIN LIGASE; POXVIRUS PROTEIN; VIRULENCE FACTOR; P-28; MOTIF; ZINC;

D O I：

10.1186/s12985-021-01640-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Zoonotic orthopoxvirus infections continue to represent a threat to human health. The disease caused by distinct orthopoxviruses differs in terms of symptoms and severity, which may be explained by the unique repertoire of virus factors that modulate the host's immune response and cellular machinery. We report here on the construction of recombinant cowpox viruses (CPXV) which either lack the host range factor p28 completely or express truncated variants of p28. We show that p28 is essential for CPXV replication in macrophages of human or mouse origin and that the C-terminal RING finger domain of p28 is necessary to allow CPXV replication in macrophages.

引用

页数：6