Determination of sparfloxacin in human urine by reversed-phase high performance liquid chromatography with nitrous acid and hydroiodic pre-column derivatization

被引：0
作者：
Du, LM
Fan, ZF
Qiao, JL
Wang, JP
机构：
[1] Shanxi Teachers Univ, Anal & Test Ctr, Lingfen 041004, Peoples R China
[2] Taiyuan Univ Technol, Coll Arts & Sci, Dept Chem, Taiyuan 030006, Peoples R China
来源：
CHINESE CHEMICAL LETTERS | 2001年 / 12卷 / 11期
关键词：
sparfloxacin; HPLC; pre-column derivatization; human urine;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%similar to 95.7% and the RSD is 1.2%similar to4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.
引用
收藏
页码：1007 / 1010
页数：4
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