Determination of sparfloxacin in human urine by reversed-phase high performance liquid chromatography with nitrous acid and hydroiodic pre-column derivatization

被引：0

作者：

Du, LM

Fan, ZF

Qiao, JL

Wang, JP

机构：

[1] Shanxi Teachers Univ, Anal & Test Ctr, Lingfen 041004, Peoples R China

[2] Taiyuan Univ Technol, Coll Arts & Sci, Dept Chem, Taiyuan 030006, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2001年 / 12卷 / 11期

关键词：

sparfloxacin; HPLC; pre-column derivatization; human urine;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%similar to 95.7% and the RSD is 1.2%similar to4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.

引用

页码：1007 / 1010

页数：4