Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems

被引：92

作者：

Marques Ventura, Sonia Patricia ^{[1
]}

Freire de Barros, Rafael Lemos ^{[2
]}

de Pinho Barbosa, Jose Murillo ^{[2
]}

Faria Soares, Cleide Mara ^{[2
]}

Lima, Alvaro Silva ^{[2
]}

Pereira Coutinho, Joao Araujo ^{[1
]}

机构：

[1] Univ Aveiro, CICECO, Dept Quim, P-3810193 Aveiro, Portugal

[2] Univ Tiradentes, Programa Posgrad Engn Proc, Aracaju, SE, Brazil

来源：

GREEN CHEMISTRY | 2012年 / 14卷 / 03期

关键词：

PHASE-DIAGRAM DATA; BIPHASIC SYSTEMS; PARTITIONING BEHAVIOR; POLYETHYLENE-GLYCOL; MOLECULAR-WEIGHT; AMINO-ACIDS; EXTRACTION; PROTEINS; IMIDAZOLIUM; SEPARATION;

D O I：

10.1039/c2gc16428k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The ability of ionic liquid-based aqueous two-phase systems (ATPS) to purify lipase produced by fermentation is here evaluated and compared against conventional PEG-based ATPS systems. Four ionic liquids, chosen after screening of a larger number of ionic liquids are evaluated, with the maximum purification and higher recovery being obtained for the systems based on [C(8)mim]Cl. It is shown that IL-based ATPS have a performance superior to PEG-based ATPS for the purification of this enzyme.

引用

页码：734 / 740

页数：7

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