Hypoxia inhibits the spontaneous calcification of bone marrow-derived mesenchymal stem cells

被引：43
作者：
Huang, Yong-Can [1 ,2 ]
Zhu, Hong-Ming [1 ]
Cai, Jia-Qin [1 ]
Huang, Yi-Zhou [1 ]
Xu, Jin [3 ]
Zhou, Yi [4 ]
Chen, Xiao-He [1 ]
Li, Xiu-Qun [1 ]
Yang, Zhi-Ming [1 ]
Deng, Li [1 ]
机构：
[1] Sichuan Univ, W China Hosp, Regenerat Med Res Ctr, Div Stem Cell & Tissue Engn, Chengdu 610041, Sichuan, Peoples R China
[2] Univ Hong Kong, Li Ka Shing Fac Med, Dept Orthopaed & Traumatol, Hong Kong 999077, Hong Kong, Peoples R China
[3] Sichuan Univ, W China Hosp, Dept Lab Med, Chengdu 610041, Sichuan, Peoples R China
[4] Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2012年 / 113卷 / 04期
基金：
中国国家自然科学基金;
关键词：
BONE MARROW; MESENCHYMAL STEM CELLS; SPONTANEOUS CALCIFICATION; NORMOXIA; HYPOXIA; OSTEOGENIC DIFFERENTIATION; STEM/PROGENITOR CELLS; STROMAL CELLS; PROLIFERATION; TRANSPLANTATION; EXPRESSION; EXPANSION; MATRIX; CANCER; REPAIR;
D O I：
10.1002/jcb.24014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are the popular seed cells for regenerative medicine, and there has been a rapid increase in the number of BM-MSC-based clinical trials. However, the safety of these cells should also be closely studied. In this study, spontaneous calcification of BM-MSCs from rats was evaluated in normoxia (20% O2) without osteogenic medium after continuous culture for 21 days; obvious mineralized nodules were observed, which were positive for Alizarin Red, collagen-I (Col-I), osteocalcin (OC) and alkaline phosphatase (ALP), and mainly consisted of C, O and Ca elements. Interestingly, hypoxia (2% O2) significantly inhibited this spontaneous calcification. In addition, the ALP and calcium content of rBM-MSCs were sharply reduced. Based on RT-PCR results, the expression of osteogenic genes (Cbfa1/Runx2, Col-I, ALP, and OC) was reduced compared to that in normoxia. These results demonstrate a natural and unique characterization of rat BM-MSCs in normoxia after continuous culture and highlight the inhibiting effects of hypoxia. Finally, this study contributes to the information regarding the application of BM-MSCs in the regeneration of various tissues. J. Cell. Biochem. 113: 14071415, 2012. (c) 2011 Wiley Periodicals, Inc.
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页码：1407 / 1415
页数：9
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