Inactivated Rabies Virus Vectored MERS-Coronavirus Vaccine Induces Protective Immunity in Mice, Camels, and Alpacas

被引：7

作者：

Chi, Hang ^{[1
]}

Wang, Yanqun ^{[2
]}

Li, Entao ^{[1
]}

Wang, Xiwen ^{[3
]}

Wang, Hualei ^{[4
]}

Jin, Hongli ^{[4
]}

Han, Qiuxue ^{[1
]}

Wang, Zhenshan ^{[1
]}

Wang, Xinyue ^{[1
]}

Zhu, Airu ^{[2
]}

Sun, Jing ^{[2
]}

Zhuang, Zhen ^{[2
]}

Zhang, Lu ^{[5
]}

Ye, Jingmeiqi ^{[6
]}

Wang, Haijun ^{[7
]}

Feng, Na ^{[1
]}

Hu, Mingda ^{[8
]}

Gao, Yuwei ^{[1
]}

Zhao, Jincun ^{[2
,9
,10
]}

Zhao, Yongkun ^{[1
]}

Yang, Songtao ^{[1
]}

Xia, Xianzhu ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China

[2] Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Hlth, State Key Lab Resp Dis,Natl Clin Res Ctr Resp Dis, Guangzhou, Peoples R China

[3] Adm Drug & Instrument Supervis & Inspect, Food & Drug Inspect Lab, Beijing, Peoples R China

[4] Jilin Univ, Coll Vet Med, Minist Educ, Key Lab Zoonosis Res, Changchun, Peoples R China

[5] Guangzhou Customs Technol Ctr, Hlth & Quarantine Lab, Guangzhou, Peoples R China

[6] Tsinghua Univ, Inst Educ, Beijing, Peoples R China

[7] Jilin Prov Northeast Tiger Garden, Jilin Wild Anim Rescue Breeding Ctr Comm, Changchun, Peoples R China

[8] Beijing Inst Biotechnol, Sate Key Lab Pathogen & Biosecur, Beijing, Peoples R China

[9] Guangzhou Med Univ, Guangzhou Eighth Peoples Hosp, Inst Infect Dis, Guangzhou, Peoples R China

[10] Guangzhou Lab, Dept Basic Res, Guangzhou, Peoples R China

来源：

FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷

基金：

中国国家自然科学基金;

关键词：

MERS-CoV; vaccine; rabies virus vector; mice; camel; alpaca; RESPIRATORY SYNDROME CORONAVIRUS; DROMEDARY CAMELS; COV INFECTION; VIRAL VECTOR; TRANSMISSION; ANTIBODIES; RESPONSES; LIVE;

D O I：

10.3389/fimmu.2022.823949

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Middle East respiratory syndrome coronavirus (MERS-CoV) is an emergent coronavirus that has caused frequent zoonotic events through camel-to-human spillover. An effective camelid vaccination strategy is probably the best way to reduce human exposure risk. Here, we constructed and evaluated an inactivated rabies virus-vectored MERS-CoV vaccine in mice, camels, and alpacas. Potent antigen-specific antibody and CD8(+) T-cell responses were generated in mice; moreover, the vaccination reduced viral replication and accelerated virus clearance in MERS-CoV-infected mice. Besides, protective antibody responses against both MERS-CoV and rabies virus were induced in camels and alpacas. Satisfyingly, the immune sera showed broad cross-neutralizing activity against the three main MERS-CoV clades. For further characterization of the antibody response induced in camelids, MERS-CoV-specific variable domains of heavy-chain-only antibody (VHHs) were isolated from immunized alpacas and showed potent prophylactic and therapeutic efficacies in the Ad5-hDPP4-transduced mouse model. These results highlight the inactivated rabies virus-vectored MERS-CoV vaccine as a promising camelid candidate vaccine.

引用

页数：15

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