Structure of the rat collagen IV promoter

被引：7

作者：

Grande, JP ^{[1
]}

Melder, DC ^{[1
]}

Kluge, DL ^{[1
]}

Wieben, ED ^{[1
]}

机构：

[1] MAYO CLIN & MAYO GRAD SCH MED,DEPT BIOCHEM & MOL BIOL,ROCHESTER,MN 55905

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1309卷 / 1-2期

关键词：

collagen IV; basement membrane; promoter; structure;

D O I：

10.1016/S0167-4781(96)00134-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a 1.6 kb clone from a rat genomic library which contains the bidirectional collagen IV promoter, flanked by exons coding for the alpha 1(IV) and alpha 2(IV) collagen chains. There are at least two transcription start sites within both the alpha 1(lV) and alpha 2(IV) collagen genes. Rat mesangial cells were transfected with chloramphenicol acetyltransferase (CAT) reporter plasmids containing segments of the promoter and 5' flanking region, in both the alpha 1(IV) and alpha 2(IV) orientations. Our results suggest that transcriptional efficiency of the bidirectional promoter is more efficient in the alpha 2(IV) direction than in the alpha 1(IV) direction.

引用

页码：85 / 88

页数：4

共 12 条

[1] BURBELO PD, 1991, J BIOL CHEM, V266, P22297
[2] ALPHA-1(IV) AND ALPHA-2(IV) COLLAGEN GENES ARE REGULATED BY A BIDIRECTIONAL PROMOTER AND A SHARED ENHANCER
BURBELO, PD
MARTIN, GR
YAMADA, Y
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) : 9679 - 9682
[3] IDENTIFICATION OF A NOVEL SEQUENCE ELEMENT IN THE COMMON PROMOTER REGION OF HUMAN COLLAGEN TYPE-IV GENES, INVOLVED IN THE REGULATION OF DIVERGENT TRANSCRIPTION
FISCHER, G
SCHMIDT, C
OPITZ, J
CULLY, Z
KUHN, K
POSCHL, E
[J]. BIOCHEMICAL JOURNAL, 1993, 292 : 687 - 695
[4] HUDSON BG, 1993, J BIOL CHEM, V268, P26033
[5] KILLEN PD, 1988, J BIOL CHEM, V263, P12310
[6] KUHN K, 1994, MATRIX BIOL, V14, P439
[7] HUMAN MESANGIAL CELLS SECRETE A GBM-DEGRADING NEUTRAL PROTEINASE AND A SPECIFIC INHIBITOR
MARTIN, J
DAVIES, M
THOMAS, G
LOVETT, DH
[J]. KIDNEY INTERNATIONAL, 1989, 36 (05) : 790 - 801
[8] REGULATION OF DIVERGENT TRANSCRIPTION OF THE GENES-CODING FOR BASEMENT-MEMBRANE TYPE-IV COLLAGEN
POLLNER, R
FISCHER, G
POSCHL, E
KUHN, K
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1990, 580 : 44 - 54
[9] Sambrook J., 2002, MOL CLONING LAB MANU
[10] DIFFERENTIAL-EFFECTS OF DNA-BINDING PROTEINS ON BIDIRECTIONAL TRANSCRIPTION FROM THE COMMON PROMOTER REGION OF HUMAN COLLAGEN TYPE-IV GENES COL4A1 AND COL4A2
SCHMIDT, C
FISCHER, G
KADNER, H
GENERSCH, E
KUHN, K
POSCHL, E
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1174 (01) : 1 - 10

← 1 2 →