Development of chiral high performance liquid chromatographic assays for the enantiomers of 8-hydroxy-(di-n-propylamino)tetralin and three 8-keto-pyrrole-substituted analogues

被引：4

作者：

Wood, SA

Pegg, SN

Simmonds, RJ

Stevenson, D

机构：

[1] PHARMACIA & UPJOHN INC, CRAWLEY RH10 3AL, W SUSSEX, ENGLAND

[2] UNIV SUNDERLAND, SCH HLTH SCI, SUNDERLAND SR1 3SD, TYNE & WEAR, ENGLAND

[3] UNIV SURREY, ROBENS INST HLTH & SAFETY, GUILDFORD GU2 5XH, SURREY, ENGLAND

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1996年 / 14卷 / 11期

关键词：

2-aminotetralin; enantiomers; factorial design;

D O I：

10.1016/0731-7085(96)01802-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two chiral HPLC methods using protein-based stationary phases for the analysis of 8-hydroxy-(di-n-propylamino)tetralin and three 8-keto-pyrrole analogues are presented. Efficient solid-phase extraction enabled quantification of 0.02 mu g ml(-1) of the 8-keto-pyrrole analogues from 500 mu l rat hepatocyte suspensions, and 0.2 mu g ml(-1) to be measured from 50 mu l of rat plasma using UV detection at 315 nm.

引用

页码：1591 / 1599

页数：9

共 15 条

[1] SYNTHESIS OF R-(+)- AND S-(-)-8-HYDROXY-2-(N,N-DIPROPYLAMINO)-[2-H-3] TETRALIN - HCL (8-OH-DPAT) A 5HT1A RECEPTOR AGONIST [J].