Piwi/piRNAs control food intake by promoting neuropeptide F expression in locusts

被引：12

作者：

Wang, Huimin ^{[1
,2
]}

Jiang, Feng ^{[1
,2
]}

Liu, Xiang ^{[3
]}

Liu, Qing ^{[3
,4
]}

Fu, Yunyun ^{[5
]}

Li, Ran ^{[1
]}

Hou, Li ^{[3
]}

Zhang, Jie ^{[1
]}

He, Jing ^{[3
]}

Kang, Le ^{[1
,2
,3
,5
]}

机构：

[1] Chinese Acad Sci, Beijing Inst Life Sci, Beijing, Peoples R China

[2] Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing, Peoples R China

[3] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China

[4] Univ Chinese Acad Sci, Sino Danish Coll, Beijing, Peoples R China

[5] Hebei Univ, Coll Life Sci, Baoding, Peoples R China

来源：

EMBO REPORTS | 2022年 / 23卷 / 03期

基金：

中国国家自然科学基金;

关键词：

feeding; neuropeptide; piRNA; PIWI protein; SMALL NONCODING RNAS; GENE-EXPRESSION; FEEDING-BEHAVIOR; MESSENGER-RNAS; DESERT LOCUST; PIWI PROTEINS; PHASE-CHANGES; PIRNA; DROSOPHILA; GENOME;

D O I：

10.15252/embr.202050851

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Animal feeding, which directly affects growth and metabolism, is an important physiological process. However, the contribution of PIWI proteins and PIWI-interacting RNAs (piRNAs) to the regulatory mechanism of animal feeding is unknown. Here, we report a novel function of Piwi and piRNAs in regulating food intake in locusts. Our study shows that the locust can serve as a representative species for determining PIWI function in insects. Knockdown of Piwi1 expression suppresses anabolic processes and reduces food consumption and body weight. The reduction in food intake by knockdown of Piwi1 expression results from decreased expression of neuropeptide NPF1 in a piRNA-dependent manner. Mechanistically, intronic piRNAs might enhance RNA splicing of NPF1 by preventing hairpin formation at the branch point sites. These results suggest a novel nuclear PIWI/piRNA-mediated mechanism that controls food intake in the locust nervous system.

引用

页数：20

共 82 条

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