Effect of Coriolis force on counter-current chromatographic separation by centrifugal partition chromatography

被引:16
作者
Ikehata, JI
Shinomiya, K
Kobayashi, K
Ohshima, H
Kitanaka, S
Ito, Y
机构
[1] Nihon Univ, Div Analyt Chem, Coll Pharm, Funabashi, Chiba 2748555, Japan
[2] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
关键词
centrifugal partition chromatography; Coriolis force; efficiency;
D O I
10.1016/j.chroma.2003.10.107
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The effect of Coriolis force on the counter-current chromatographic separation was studied using centrifugal partition chromatography (CPC) with four different two-phase solvent systems including n-hexane-acetonitrile (ACN); tert-butyl methyl ether (MtBE)-aqueous 0.1% trifluoroacetic acid (TFA) (1:1); MtBE-ACN-aqueous 0.1% TFA (2:2:3); and 12.5% (w/w) polyethylene glycol (PEG) 1000-12.5% (w/w) dibasic potassium phosphate. Each separation was performed by eluting either the upper phase in the ascending mode or the lower phase in the descending mode, each in clockwise (CW) and counterclockwise column rotation. Better partition efficiencies were attained by the CW rotation in both mobile phases in all the two-phase solvent systems examined. The mathematical analysis also revealed the Coriolis force works favorably under the CW column rotation for both mobile phases. The overall results demonstrated that the Coriolis force produces substantial effects on CPC separation in both organic-aqueous and aqueous-aqueous two-phase systems. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:169 / 175
页数:7
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