Identification of a cadmium-induced gene in Mycobacterium bovis and Mycobacterium tuberculosis

被引：1

作者：

Hotter, GS ^{[1
]}

Wilson, T ^{[1
]}

Collins, DM ^{[1
]}

机构：

[1] AgRes, Wallaceville Anim Res Ctr, Upper Hutt, New Zealand

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 200卷 / 02期

关键词：

Mycobacterium tuberculosis; cadmium; gene expression;

D O I：

10.1111/j.1574-6968.2001.tb10707.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 17-kDa protein (CadI) was induced by cadmium in Mycobacterium bovis and Mycobacterium tuberculosis. Comparison of the N-terminal sequence from M. bovis CadI with the annotated M. tuberculosis genome database identified Rv2641 as the encoding gene. Long and short promoter fragments from M. bovis cadI were fused to the lacZ reporter gene in pYUB76. Only the long fragment directed cadmium-inducible activity when electroporated into M. bovis. The cadI promoter has potential for both constitutive and inducible expression studies in M. bovis and M. tuberculosis. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：151 / 155

页数：5

共 15 条

[1] IDENTIFICATION OF EXPRESSION SIGNALS OF THE MYCOBACTERIOPHAGE-BXB1, MYCOBACTERIOPHAGE-L1 AND MYCOBACTERIOPHAGE-TM4 USING THE ESCHERICHIA-MYCOBACTERIUM SHUTTLE PLASMID-PYUB75 AND PLASMID-PYUB76 DESIGNED TO CREATE TRANSLATIONAL FUSIONS TO THE LACZ GENE
BARLETTA, RG
KIM, DD
SNAPPER, SB
BLOOM, BR
JACOBS, WR
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 : 23 - 30
[2] Fosfomycin resistance protein (FosA) is a manganese metalloglutathione transferase related to glyoxalase I and the extradiol dioxygenases
Bernat, BA
Laughlin, LT
Armstrong, RN
[J]. BIOCHEMISTRY, 1997, 36 (11) : 3050 - 3055
[3] IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS
BLUM, H
BEIER, H
GROSS, HJ
[J]. ELECTROPHORESIS, 1987, 8 (02) : 93 - 99
[4] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Cole, ST
Brosch, R
Parkhill, J
Garnier, T
Churcher, C
Harris, D
Gordon, SV
Eiglmeier, K
Gas, S
Barry, CE
Tekaia, F
Badcock, K
Basham, D
Brown, D
Chillingworth, T
Connor, R
Davies, R
Devlin, K
Feltwell, T
Gentles, S
Hamlin, N
Holroyd, S
Hornby, T
Jagels, K
Krogh, A
McLean, J
Moule, S
Murphy, L
Oliver, K
Osborne, J
Quail, MA
Rajandream, MA
Rogers, J
Rutter, S
Seeger, K
Skelton, J
Squares, R
Squares, S
Sulston, JE
Taylor, K
Whitehead, S
Barrell, BG
[J]. NATURE, 1998, 393 (6685) : 537 - +
[5] Metallothionein: An intracellular protein to protect against cadmium toxicity
Klaassen, CD
Liu, J
Choudhuri, S
[J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1999, 39 : 267 - 294
[6] ROLE OF ANTIOXIDANTS IN PROTECTING CELLULAR DNA FROM DAMAGE BY OXIDATIVE STRESS
MARTINS, EAL
CHUBATSU, LS
MENEGHINI, R
[J]. MUTATION RESEARCH, 1991, 250 (1-2): : 95 - 101
[7] CONSEQUENCES OF CADMIUM TOXICITY IN RAT HEPATOCYTES - MITOCHONDRIAL DYSFUNCTION AND LIPID-PEROXIDATION
MULLER, L
[J]. TOXICOLOGY, 1986, 40 (03) : 285 - 295
[8] Distribution of thiols in microorganisms: Mycothiol is a major thiol in most actinomycetes
Newton, GL
Arnold, K
Price, MS
Sherrill, C
Delcardayre, SB
Aharonowitz, Y
Cohen, G
Davies, J
Fahey, RC
Davis, C
[J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (07) : 1990 - 1995
[9] ISOLATION AND CHARACTERIZATION OF EFFICIENT PLASMID TRANSFORMATION MUTANTS OF MYCOBACTERIUM-SMEGMATIS
SNAPPER, SB
MELTON, RE
MUSTAFA, S
KIESER, T
JACOBS, WR
[J]. MOLECULAR MICROBIOLOGY, 1990, 4 (11) : 1911 - 1919
[10] An inducible expression system permitting the efficient purification of a recombinant antigen from Mycobacterium smegmatis
Triccas, JA
Parish, T
Britton, WJ
Gicquel, B
[J]. FEMS MICROBIOLOGY LETTERS, 1998, 167 (02) : 151 - 156

← 1 2 →