Determination of morphine and norlaudanosoline in murine brain regions by dispersive liquid-liquid micro-extraction and liquid chromatograpy-electrochemical detection

被引：0

作者：

Nagler, Joachim ^{[1
]}

Schriever, Sonja C. ^{[2
]}

Romanov, Artem ^{[3
]}

Vogt-Weisenhorn, Daniela ^{[3
,4
]}

Wurst, Wolfgang ^{[3
,4
,5
]}

Pfluger, Paul T. ^{[2
]}

Schramm, Karl-Werner ^{[1
,6
]}

机构：

[1] Helmholtz Ctr Munich German Res Ctr Environm Hlth, Mol EXpos MEX, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

[2] Helmholtz Ctr Munich German Res Ctr Environm Hlth, Res Unit NeuroBioloy Diabet NBD, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

[3] Helmholtz Ctr Munich German Res Ctr Environm Hlth, Inst Dev Genet IDG, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

[4] Tech Univ Munich Weihenstephan, Dev Genet, Helmholtz Zentrum Munchen, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

[5] German Ctr Neurodegenerat Dis DZNE, Site Munich, Feodor Lynen Str 17, D-81377 Munich, Germany

[6] Tech Univ Munich, Dept Biowissensch Grundlagen, Wissenschaftszentrum Weihenstephan Ernahrung Land, Weihenstephaner Steig 23, D-85350 Freising Weihenstephan, Germany

来源：

NEUROCHEMISTRY INTERNATIONAL | 2021年 / 150卷

关键词：

Liquid chromatography; Electrochemical detection; Dispersive liquid-liquid microextraction; Morphine; Norlaudanosoline; Murine brain regions; ENDOGENOUS MORPHINE; TETRAHYDROPAPAVEROLINE; MICROEXTRACTION; METABOLITES; PRECONCENTRATION; LOCALIZATION; DOPAMINE;

D O I：

10.1016/j.neuint.2021.105174

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Morphine can be synthesized endogenously by mammals from dopamine via the intermediate norlaudanosoline. Previously, both compounds have been detected separately in whole brains of mice and brain regions of rats, and in urine of humans. Here, we report a novel method for the analysis of both compounds in single murine brain regions. Initially, a variant of dispersive liquid-liquid microextraction was established by using methanol as an extractant, cyclohexane as solvent, and tributylphosphate as disperser. The extraction method was applied to murine brain regions homogenized with perchloric acid while the subsequent detection was carried out by HPLC with electrochemical detection. In the thalamus of C57Bl/6J mice (n = 3, male, age 4-8 months), morphine and norlaudanosoline could be detected at levels of 19 +/- 3.9 and 7.2 +/- 2.3 pg/mg, respectively. Overall, we provide a novel method for the simultaneous extraction and detection of both morphine and norlaudanosoline in single murine brain regions.

引用

页数：8

共 29 条

[1] A simple and sensitive high-performance liquid chromatography-electrochemical detection assay for the quantitative determination of monoamines and respective metabolites in six discrete brain regions of mice [J].