Quantitative detection of species-specific DNA in feedstuffs and fish meals

被引：33

作者：

Krcmar, P ^{[1
]}

Rencova, E ^{[1
]}

机构：

[1] Vet Res Inst, CS-62132 Brno, Czech Republic

来源：

JOURNAL OF FOOD PROTECTION | 2005年 / 68卷 / 06期

关键词：

D O I：

10.4315/0362-028X-68.6.1217

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species-derived material in concentrate feed mixtures and fish meals.

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页码：1217 / 1221

页数：5

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