ORAChromatography and Total Phenolics Content of Peanut Root Extracts

被引:4
|
作者
Holland, Kevin W. [1 ]
Balota, Maria [2 ]
Eigel, William N., III [1 ]
Mallikarjunan, Parameswarakumar [3 ]
Tanko, James M. [4 ]
Zhou, Kequan [5 ]
O'Keefe, Sean F. [1 ]
机构
[1] Virginia Tech, Dept Food Sci & Technol, Blacksburg, VA 24061 USA
[2] Tidewater Agr Res & Extens Ctr, Suffolk, VA 23437 USA
[3] Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24061 USA
[4] Virginia Tech, Dept Chem, Blacksburg, VA 24061 USA
[5] Wayne State Univ, Dept Nutr & Food Sci, Detroit, MI 48202 USA
关键词
antioxidant; ORAC; peanut roots; phytoalexin; total phenolics; RADICAL SCAVENGING COMPOUNDS; FOLIN-CIOCALTEU REAGENT; HPLC-DPPH METHOD; STILBENE PHYTOALEXINS; ARACHIS-HYPOGAEA; ANTIOXIDANTS; CAPACITY; ACCUMULATION; RESVERATROL; FOODS;
D O I
10.1111/j.1750-3841.2011.02069.x
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
A large number of compounds have been reported in peanut plants. Many of these compounds are phytoalexins, which are produced by plants experiencing environmental stress and often exhibit antioxidant activity. It is difficult to determine which of the many compounds has the greatest impact on total antioxidant capacity in a mixture. The objectives of this research were to examine the oxygen-radical absorbing capacity (ORAC) value and total phenolic contents of peanut root extracts and peanut root extract fractions collected via HPLC. Peanut roots were extracted from four different cultivars (Brantley, NC-12, Phillips, and Wilson) with 70% aqueous ethanol with ultrasonic assistance. Each cultivar was sampled in duplicate. The extracts were fractionated into 18 3-min fractions by HPLC using a C-18 column. Fractions and crude extracts were freeze dried. ORAC values and total phenolic content were then determined for all fractions and crude extracts. Fractions had a significant effect on the mu M TE/mg gallic acid equivalents (GAE). ORAC values ranged from -46.89 mu M TE to 185 mu M TE in HPLC fractions. ORAChromatography can be used to focus on antioxidants in complex samples.
引用
收藏
页码:C380 / C384
页数:5
相关论文
共 50 条
  • [21] Total antioxidant capacity and phenolics content in fresh apricots
    Leccese, A.
    Bartolini, S.
    Viti, R.
    ACTA ALIMENTARIA, 2008, 37 (01) : 65 - 76
  • [22] The Total Phenolics Content of Fruit & Vegetable Extracts Does Not Correlate with Activation of the Anti-Oxidant Response in Human Cells
    Orena, Stephen Joseph
    Owen, Jennifer
    Jin, Fuxia
    Fabian, Morgan
    Gillitt, Nicholas
    Zeisel, Steven
    FASEB JOURNAL, 2016, 30
  • [23] Comparative assessment of total phenolics content and in vitro antioxidant capacity variations of leaf extracts of Origanum grossii and Thymus pallidus
    Zejli, Hind
    EL Amrani, Belkacem
    Metouekel, Amira
    Bousseraf, Fatima Zafra
    Fitat, Aziza
    Taleb, Mustapha
    Abdellaoui, Abdelfattah
    MOROCCAN JOURNAL OF CHEMISTRY, 2024, 12 (01): : 361 - 375
  • [24] TOTAL PHENOLICS, TOTAL TANNINS AND ANTIOXIDANT ACTIVITY OF CASSIA FISTULA L. EXTRACTS OF BARK, STEM, LEAF AND ROOT UNDER DIFFERENT AGE CLASSES
    Tzekiat, Lai
    Chiang, Liew Kang
    ASIAN JOURNAL OF PHARMACEUTICAL RESEARCH AND HEALTH CARE, 2013, 5 (02) : 52 - 57
  • [25] Total phenolics concentration and antioxidant potential of extracts of medicinal plants of Pakistan
    Saleem, A
    Ahotupa, M
    Pihlaja, K
    ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 2001, 56 (11-12): : 973 - 978
  • [26] Phenolics content, in vitro antioxidant and anticholinesterase activities of combined berry extracts
    Oancea, Simona
    Calin, Felicia
    ROMANIAN BIOTECHNOLOGICAL LETTERS, 2018, 23 (05): : 14025 - 14034
  • [27] Correlation Study of Biological Activity with Quercetin and Phenolics Content in Onion Extracts
    Olszowy-Tomczyk, Malgorzata
    Garbaczewska, Sylwia
    Wianowska, Dorota
    MOLECULES, 2022, 27 (23):
  • [28] Effects of Boiling and Roasting Treatments on the Content of Total Phenolics and Flavonoids and the Antioxidant Activity of Peanut (Arachis hypogaea L.) Pod Shells
    Hassan, Amro B.
    Al Maiman, Salah A.
    Alshammari, Ghedeir M.
    Mohammed, Mohammed A.
    Alhuthayli, Haya F.
    Ahmed, Isam A. Mohamed
    Alfawaz, Mohammed A.
    Yagoub, Abu ElGasim A.
    Fickak, Adil
    Osman, Magdi A.
    PROCESSES, 2021, 9 (09)
  • [29] Total phenolics, non-tannin phenolics and total tannin content of commonly available forages for ruminants in Tamil Nadu
    Bharathidhasan, A.
    Viswanathan, K.
    Balakrishnan, V.
    RANGE MANAGEMENT AND AGROFORESTRY, 2013, 34 (02) : 205 - 208
  • [30] Ascorbic Acid, Total Phenolics and Antioxidant Activity of Guava Leaf Extracts
    Iamjud, K.
    Banyen, N.
    Boonprakob, U.
    Thaipong, K.
    INTERNATIONAL SYMPOSIUM ON TROPICAL AND SUBTROPICAL FRUITS, 2014, 1024 : 367 - 372