Pelagivirga sediminicola gen. nov., sp nov isolated from the Bohai Sea

A Gram-stain-negative bacterium, strain BH-SD19(T), that was isolated from a marine sediment sample collected from the Bohai Sea, was subjected to a polyphasic taxonomic study. Cells of BH-SD19(T) are non-flagellated, non-gliding, oval-shaped rods, 0.5-1.0 mu m wide and 1.0-2.0 mu m long. BH-SD19(T) is strictly aerobic, and oxidase-and catalase-positive. Growth occurs at 15-40 degrees C (optimum 35 degrees C), at pH 6.0-8.5 (optimum 7.0-7.5) and with 1-10% (w/v) NaCl (optimum 2 %). The predominant fatty acids are C-19:0cyclo omega 8c (46.5 %), C-16:0 (20.3 %) and C-18:1 omega 7c and/or C-18:1 omega 6c (10.6 %). The major respiratory quinone is Q-10. The major polar lipids are phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content is 64.0 mol%. BH-SD19T shows the highest 16S rRNA sequence similarity to Pontibaca methylaminivorans (95.2 %) and strains of species of the genus Roseovarius (93.4-95.2 %). Sequence similarity values between BH-SD19(T) and other phylogenetically related species are all below 95.0 %. Phylogenetic trees based on 16S rRNA gene sequences indicate that BH-SD19(T) forms a distinct lineage and does not join any known genera in the trees. Phenotypic, chemotaxonomic and phylogenetic data indicate that BH-SD19(T) represents a novel genus and species in the family Rhodobacteraceae, for which the name Pelagivirga sediminicola gen. nov., sp. nov. is proposed. The type strain is BH-SD19T (=CCTCC AB 2017074(T) = KCTC 62202(T)).