Detection of the multidrug resistance marker P-glycoprotein by immunohistochemistry in malignant lung tumours

被引:8
作者
Beer, TW
Rowlands, DC
Crocker, J
机构
[1] GEN HOSP,DEPT HISTOPATHOL,BIRMINGHAM B4 6NH,W MIDLANDS,ENGLAND
[2] BIRMINGHAM HEARTLANDS HOSP,DEPT HISTOPATHOL,BIRMINGHAM B9 5ST,W MIDLANDS,ENGLAND
关键词
multidrug resistance; P-glycoprotein; lung carcinoma;
D O I
10.1136/thx.51.5.526
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background - The multidrug resistance marker P-glycoprotein (P-gp) was studied immunohistochemically in 78 primary malignant lung rumours. P-gp is a 170 kD transmembrane ATP dependent drug efflux pump which has been shown to be important in the resistance of some tumours to chemotherapy. Certain normal tissues express P-gp and tumours derived from these tissues are often insensitive to cytotoxic agents, showing raised P-gp levels innately or following chemotherapy or radiotherapy. Methods - Samples from 78 patients undergoing surgery for primary malignant lung tumours were snap frozen and stained immunohistochemically using the monoclonal antibody C219 which reacts with a P-gp epitope. None of the study group had received chemotherapy or radiotherapy before surgery was performed. Results - Twenty seven of the 78 lung tumours (34.6%) showed immunohistochemically detectable levels of P-gp which varied with tumour type; 17 of 54 squamous cell carcinomas (31.5%), seven of 15 adenocarcinomas (46.7%), and neither of two small cell carcinomas showing positive staining. In six of seven cases normal respiratory epithelium present showed the presence of P-gp. Conclusions - P-gp is immunohistochemically detectable in frozen tissue from a proportion of malignant lung rumours before exposure to radiotherapy or drugs associated with multidrug resistance. It may have a role in tumour resistance to cytotoxic drugs, but further clinical studies will be required to evaluate any correlation between P-gp levels and response to treatment.
引用
收藏
页码:526 / 529
页数:4
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[41]   Characterization of functional assays of multidrug resistance P-glycoprotein transport activity [J].
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[46]   Osteoblastic differentiation and P-glycoprotein multidrug resistance in a murine osteosarcoma model [J].
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Murata, H ;
Suginoshita, T ;
Hirata, M ;
Hashiguchi, S ;
Ashihara, T ;
Gebhardt, MC ;
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[48]   Multidrug resistance mediated by overexpression of P-glycoprotein in human osteosarcoma in vivo [J].
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Tamaoki, N ;
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[50]   Brain Microvascular P-Glycoprotein and a Revised Model of Multidrug Resistance in Brain [J].
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