Innate lymphoid cells regulate intestinal epithelial cell glycosylation

被引：423

作者：

Goto, Yoshiyuki ^{[1
,2
,3
]}

Obata, Takashi ^{[1
,3
]}

Kunisawa, Jun ^{[1
,4
,5
]}

Sato, Shintaro ^{[1
,2
]}

Ivanov, Ivaylo I. ^{[6
]}

Lamichhane, Aayam ^{[1
]}

Takeyama, Natsumi ^{[1
,7
]}

Kamioka, Mariko ^{[1
]}

Sakamoto, Mitsuo ^{[3
]}

Matsuki, Takahiro ^{[8
]}

Setoyama, Hiromi ^{[8
]}

Imaoka, Akemi ^{[8
]}

Uematsu, Satoshi ^{[9
,10
]}

Akira, Shizuo ^{[11
]}

Domino, Steven E. ^{[12
]}

Kulig, Paulina ^{[13
]}

Becher, Burkhard ^{[13
]}

Renauld, Jean-Christophe ^{[14
,15
]}

Sasakawa, Chihiro ^{[7
,16
,17
]}

Umesaki, Yoshinori ^{[8
]}

Benno, Yoshimi ^{[18
]}

Kiyono, Hiroshi ^{[1
,2
,5
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Mucosal Immunol, Tokyo 1088639, Japan

[2] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol, Kawaguchi, Saitama 3320012, Japan

[3] RIKEN, BioResource Ctr, Microbe Div Japan Collect Microorganisms, Tsukuba, Ibaraki 3050074, Japan

[4] Natl Inst Biomed Innovat, Lab Vaccine Mat, Osaka 5670085, Japan

[5] Univ Tokyo, Inst Med Sci, Div Mucosal Immunol, Int Res & Dev Ctr Mucosal Vaccines, Tokyo 1088639, Japan

[6] Columbia Univ, Med Ctr, Dept Microbiol & Immunol, New York, NY 10032 USA

[7] Nippon Inst Biol Sci, Tokyo 1980024, Japan

[8] Yakult Cent Inst, Tokyo 1868650, Japan

[9] Univ Tokyo, Inst Med Sci, Int Res & Dev Ctr Mucosal Vaccines, Div Innate Immune Regulat, Tokyo 1088639, Japan

[10] Chiba Univ, Sch Med, Dept Mucosal Immunol, Chuou Ku, Chiba 2608670, Japan

[11] Osaka Univ, WPI Immunol Frontier Res Ctr, Lab Host Def, Osaka 5650871, Japan

[12] Univ Michigan, Med Ctr, Cellular & Mol Biol Program, Dept Obstet & Gynecol, Ann Arbor, MI 48109 USA

[13] Univ Zurich, Inst Expt Immunol, CH-8057 Zurich, Switzerland

[14] Ludwig Inst Canc Res, B-1200 Brussels, Belgium

[15] Catholic Univ Louvain, B-1200 Brussels, Belgium

[16] Univ Tokyo, Inst Med Sci, Div Bacterial Infect, Tokyo 1088639, Japan

[17] Chiba Univ, Med Mycol Res Ctr, Chiba 2608673, Japan

[18] RIKEN, Innovat Ctr, Benno Lab, Wako, Saitama 3510198, Japan

来源：

SCIENCE | 2014年 / 345卷 / 6202期

基金：

日本科学技术振兴机构; 美国国家卫生研究院;

关键词：

SEGMENTED FILAMENTOUS BACTERIA; ROR-GAMMA-T; ENTERICA SEROTYPE TYPHIMURIUM; COMMENSAL MICROFLORA; HOST; FUT2; MICROBIOTA; DIFFERENTIATION; SUSCEPTIBILITY; PATHWAY;

D O I：

10.1126/science.1254009

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fucosylation of intestinal epithelial cells, catalyzed by fucosyltransferase 2 (Fut2), is a major glycosylation mechanism of host-microbiota symbiosis. Commensal bacteria induce epithelial fucosylation, and epithelial fucose is used as a dietary carbohydrate by many of these bacteria. However, the molecular and cellular mechanisms that regulate the induction of epithelial fucosylation are unknown. Here, we show that type 3 innate lymphoid cells (ILC3) induced intestinal epithelial Fut2 expression and fucosylation in mice. This induction required the cytokines interleukin-22 and lymphotoxin in a commensal bacteria-dependent and -independent manner, respectively. Disruption of intestinal fucosylation led to increased susceptibility to infection by Salmonella typhimurium. Our data reveal a role for ILC3 in shaping the gut microenvironment through the regulation of epithelial glycosylation.

引用

页码：1310 / +

页数：12

共 56 条

[1] Homeostatic Regulation of Salmonella-Induced Mucosal Inflammation and Injury by IL-23 [J].

Awoniyi, Muyiwa ;

Miller, Samuel I. ;

Wilson, Christopher B. ;

Hajjar, Adeline M. ;

Smith, Kelly D. .

PLOS ONE, 2012, 7 (05)

[2] A model of host-microbial interactions in an open mammalian ecosystem [J].

Bry, L ;

Falk, PG ;

Midtvedt, T ;

Gordon, JI .

SCIENCE, 1996, 273 (5280) :1380-1383

[3] Innate lymphoid cells drive interleukin-23-dependent innate intestinal pathology [J].

Buonocore, Sofia ;

Ahern, Philip P. ;

Uhlig, Holm H. ;

Ivanov, Ivaylo I. ;

Littman, Dan R. ;

Maloy, Kevin J. ;

Powrie, Fiona .

NATURE, 2010, 464 (7293) :1371-1375

[4] Salmonella enterica serotype Typhimurium Std fimbriae bind terminal α(1,2)fucose residues in the cecal mucosa [J].

Chessa, Daniela ;

Winter, Maria G. ;

Jakomin, Marcello ;

Baeumler, Andreas J. .

MOLECULAR MICROBIOLOGY, 2009, 71 (04) :864-875

[5] Bacterial glycans: Key mediators of diverse host immune responses [J].

Comstock, Laurie E. ;

Kasper, Dennis L. .

CELL, 2006, 126 (05) :847-850

[6] Human symbionts use a host-like pathway for surface fucosylation [J].

Coyne, MJ ;

Reinap, B ;

Lee, MM ;

Comstock, LE .

SCIENCE, 2005, 307 (5716) :1778-1781

[7] HABITAT, SUCCESSION, ATTACHMENT, AND MORPHOLOGY OF SEGMENTED, FILAMENTOUS MICROBES INDIGENOUS TO MURINE GASTROINTESTINAL-TRACT [J].

DAVIS, CP ;

SAVAGE, DC .

INFECTION AND IMMUNITY, 1974, 10 (04) :948-956

[8] Abnormal Development of Peripheral Lymphoid Organs in Mice Deficient in Lymphotoxin [J].

De Togni, Pietro ;

Goellner, Josphe ;

Ruddle, Nancy H. ;

Streeter, Philip R. ;

Fick, Andrea ;

Mariathasan, Sanjeev ;

Smith, Stacy C. ;

Carison, Rebecca ;

Shonnick, Laurie P. ;

strauss-Schoenberger, Jena ;

Russell, John H. ;

Karr, Robert ;

Chaplin, David D. .

JOURNAL OF IMMUNOLOGY, 2014, 192 (05) :2010-2014

[9] Deficiency of reproductive tract α(1,2)fucosylated glycans and normal fertility in mice with targeted deletions of the FUT1 or FUT2 α(1,2)fucosyltransferase locus [J].

Domino, SE ;

Zhang, L ;

Gillespie, PJ ;

Saunders, TL ;

Lowe, JB .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (24) :8336-8345

[10] An essential function for the nuclear receptor RORγt in the generation of fetal lymphoid tissue inducer cells [J].

Eberl, G ;

Marmon, S ;

Sunshine, MJ ;

Rennert, PD ;

Choi, YW ;

Littman, DR .

NATURE IMMUNOLOGY, 2004, 5 (01) :64-73

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