A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite

被引：3059
作者：
Miranda, KM [1 ]
Espey, MG [1 ]
Wink, DA [1 ]
机构：
[1] NCI, Radiat Biol Branch, Bethesda, MD 20892 USA
来源：
NITRIC OXIDE-BIOLOGY AND CHEMISTRY | 2001年 / 5卷 / 01期
关键词：
Griess assay; vanadium(III) reduction; nitric oxide; nitrate; nitrite;
D O I：
10.1006/niox.2000.0319
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Numerous methods are available for measurement of nitrate (NO3-). However, these assays can either be time consuming or require specialized equipment (e.g., nitrate reductase, chemiluminescent detector). We have developed a method for simultaneous evaluation of nitrate and nitrite concentrations in a microtiter plate format. The principle of this assay is reduction of nitrate by vanadium(III) combined with detection by the acidic Griess reaction. This assay is sensitive to 0.5 muM NO3- and is useful in a variety of fluids including cell culture media, serum, and plasma. S-Nitrosothiols and L-arginine derivatives were found to be potential interfering agents. However, these compounds are generally minor constituents of biological fluids relative to the concentration of nitrate/nitrite. This report introduces a new, convenient assay for the stable oxidation products of nitrogen oxide chemistry in biological samples. (C) zool Academic Press.
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收藏
页码：62 / 71
页数：10
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