Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography

被引：54

作者：

Kawakami, Keisuke

Iwai, Masako

Ikeuchi, Masahiko

Kamiya, Nobuo

Shen, Jian-Ren ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Nat Sci & Technol, Fac Sci, Div Biosci, Okayama 7008530, Japan

[2] Tokyo Univ Sci, Fac Sci & Technol, Dept Appl Biol Sci, Chiba 2788510, Japan

[3] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci Biol, Tokyo 1538902, Japan

[4] Osaka City Univ, Grad Sch Sci, Dept Chem, Osaka 5588585, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 25期

关键词：

photosystem II; PsbY; crystal structure; mutant; crystallization; membrane proteins;

D O I：

10.1016/j.febslet.2007.09.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem 11 (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII dimer. The results obtained showed that PsbY is located in the periphery of PSII close to the alpha- and beta-subunits of cytochrome b559, which corresponded to an unassigned helix in the 3.7 angstrom structure of T. vulcanus or helix X2 in the 3.0 angstrom structure of T. elongatus. Our results also indicated that the C-terminal loop of PsbY is protruded toward the stromal side, instead of the lumenal side predicted previously. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4983 / 4987

页数：5

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