A vernalization protocol for obtaining progenies from regenerated and transgenic tall fescue plants

被引：8
|
作者：
Wang, ZY [1 ]
Bell, J [1 ]
Scott, M [1 ]
机构：
[1] Samuel Roberts Noble Fdn Inc, Forage Biotechnol Grp, Ardmore, OK 73401 USA
来源：
PLANT BREEDING | 2003年 / 122卷 / 06期
关键词：
Festuca arundinacea; forage and turf grass; plant regeneration; transgenic plants; vernalization;
D O I：
10.1111/j.1439-0523.2003.00879.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Tall fescue is an important outcrossing forage and turf grass species that requires vernalization to flower. A reproducible protocol was developed for vernalization of regenerated, transgenic and seed-derived tall fescue plants. Following the vernalization scheme that involved gradual changes of temperature and daylength, seeds were routinely produced from vernalized plants under greenhouse conditions. Molecular analyses of progenies obtained from crosses between transgenic and seed-derived plants revealed stable meiotic transmission of transgenes following Mendelian inheritance in transgenic tall fescue.
引用
收藏
页码：536 / 538
页数：3
相关论文
共 18 条
  • [1] Field performance of transgenic tall fescue (Festuca arundinacea Schreb.) plants and their progenies
    Wang, ZY
    Scott, M
    Bell, J
    Hopkins, A
    Lehmann, D
    THEORETICAL AND APPLIED GENETICS, 2003, 107 (03) : 406 - 412
  • [2] Field performance of transgenic tall fescue (Festuca arundinacea Schreb.) plants and their progenies
    Z. Y. Wang
    M. Scott
    J. Bell
    A. Hopkins
    D. Lehmann
    Theoretical and Applied Genetics, 2003, 107 : 406 - 412
  • [3] MEIOTIC AND ISOZYMIC CHARACTERIZATION OF PLANTS REGENERATED FROM EUPLOID AND SELFED MONOSOMIC TALL FESCUE EMBRYOS
    DAHLEEN, LS
    EIZENGA, GC
    THEORETICAL AND APPLIED GENETICS, 1990, 79 (01) : 39 - 44
  • [4] Regeneration of fertile transgenic tall fescue plants with a stable highly expressed foreign gene
    Kuai, B
    Dalton, SJ
    Bettany, AJE
    Morris, P
    PLANT CELL TISSUE AND ORGAN CULTURE, 1999, 58 (02) : 149 - 154
  • [5] Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants
    Z.Y. Wang
    X.D. Ye
    J. Nagel
    I. Potrykus
    G. Spangenberg
    Plant Cell Reports, 2001, 20 : 213 - 219
  • [6] Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants
    Wang, ZY
    Ye, XD
    Nagel, J
    Potrykus, I
    Spangenberg, G
    PLANT CELL REPORTS, 2001, 20 (03) : 213 - 219
  • [7] REPRODUCTIVE DISTURBANCES AND PHOSPHOGLUCOISOMERASE INSTABILITY IN FESTUCA-ARUNDINACEA (TALL FESCUE) PLANTS REGENERATED FROM CALLUS AND CELL-SUSPENSION CULTURES
    GARCIA, A
    DALTON, SJ
    HUMPHREYS, MO
    HEREDITY, 1994, 73 : 355 - 362
  • [8] Development of transgenic tall fescue plants from mature seed-derived callus via Agrobacterium-mediated transformation
    Lee, SH
    Lee, DG
    Woo, HS
    Lee, BH
    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 2004, 17 (10): : 1390 - 1394
  • [9] Overexpression of a Ran GTPase homologous gene, FaRan from tall fescue, in transgenic Arabidopsis
    Lue, S. H.
    Fan, Y. L.
    Jin, C. X.
    BIOLOGIA PLANTARUM, 2011, 55 (02) : 331 - 334
  • [10] Responses of MxPPO overexpressing transgenic tall fescue plants to two diphenyl-ether herbicides, oxyfluorfen and acifluorfen
    Ki-Won Lee
    Nagib Ahsan
    Sang-Hoon Lee
    Dong-Gi Lee
    Kyung-Hee Kim
    Iftekhar Alam
    Suk-Yoon Kwon
    Jin-Seog Kim
    Kyoungwhan Back
    Sung Sil Lee
    Byung-Hyun Lee
    Acta Physiologiae Plantarum, 2008, 30
12