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Cytocompatibility and early osseointegration of nanoTiO2-modified Ti-24 Nb-4 Zr-7.9 Sn surfaces
被引:28
作者:
Liu, X. H.
[1
]
Wu, L.
[1
]
Ai, H. J.
[1
]
Han, Y.
[2
]
Hu, Y.
[1
]
机构:
[1] China Med Univ, Sch Stomatol, Dept Prosthodont, Shenyang 110001, Peoples R China
[2] Xi An Jiao Tong Univ, State Key Lab Mech Behav Mat, Xian 710049, Peoples R China
来源:
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
|
2015年
/
48卷
基金:
中国国家自然科学基金;
关键词:
Nanostructures;
Surface modification;
beta-Type titanium alloy;
Cytocompatibility;
Histocompatibility;
Osseointegration;
IN-VITRO CYTOTOXICITY;
TITANIUM IMPLANTS;
COATINGS;
OSTEOCONDUCTIVITY;
HYDROXYAPATITE;
ALLOYS;
GROWTH;
PROLIFERATION;
BEHAVIOR;
D O I:
10.1016/j.msec.2014.12.011
中图分类号:
TB3 [工程材料学];
R318.08 [生物材料学];
学科分类号:
0805 ;
080501 ;
080502 ;
摘要:
This study aimed to evaluate the cytocompatibility and early osseointegration of Ti-24 Nb-4 Zr-7.9 Sn (Ti-2448) surfaces that were modified with a nanoscale TiO2 coating. The coating was fabricated using a hydrothermal synthesis method to generate nanoTiO(2)/H-2448. The surface characteristics of the samples were evaluated using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS) and X-ray diffraction (XRD). The cytotoxicity of the fabricated nanoTiO(2)/Ti-2448 was determined using MTT assays. The proliferation and alkaline phosphatase (ALP) activity of MC3T3-E1 osteoblasts cultured on nanoTiO(2)/Ti-2448 were compared with those cultured on Ti-2448. Disk-shaped implants were placed in Wistar rats. The histological sections were stained with haematoxylin and eosin (HE), and the histocompatibility was analysed at 4 and 12 weeks post-implantation. Cylindrical implants were embedded in Japanese white rabbits, and the histological sections were stained with HE and anti-TGF-beta 1 to evaluate the histocompatibility and early osseointegration at 4, 12 and 26 weeks post-implantation. NanoTiO(2)/Ti-2448 exhibited a rougher surface than did Ti-2448. NanoTiO(2)/Ti-2448 was determined to be non-cytotoxic. More osteoblasts and higher ALP activity were observed for nanoTiO(2)/Ti-2448 than Ti-2448 < 0.05), Few inflammatory cells were detected around nanoTiO(2)/Ti-2448, and the expression of TGF-beta 1 on nanoTiO(2)/Ti-2448 peaked at earlier time than that on Ti-2448. The results indicate that the cytocompatibility and early osseointegration were enhanced by the nanoTiO(2) coating. (C) 2014 Elsevier B.V. All rights reserved.
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页码:256 / 262
页数:7
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