Cytocompatibility and early osseointegration of nanoTiO2-modified Ti-24 Nb-4 Zr-7.9 Sn surfaces

被引:28
作者
Liu, X. H. [1 ]
Wu, L. [1 ]
Ai, H. J. [1 ]
Han, Y. [2 ]
Hu, Y. [1 ]
机构
[1] China Med Univ, Sch Stomatol, Dept Prosthodont, Shenyang 110001, Peoples R China
[2] Xi An Jiao Tong Univ, State Key Lab Mech Behav Mat, Xian 710049, Peoples R China
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2015年 / 48卷
基金
中国国家自然科学基金;
关键词
Nanostructures; Surface modification; beta-Type titanium alloy; Cytocompatibility; Histocompatibility; Osseointegration; IN-VITRO CYTOTOXICITY; TITANIUM IMPLANTS; COATINGS; OSTEOCONDUCTIVITY; HYDROXYAPATITE; ALLOYS; GROWTH; PROLIFERATION; BEHAVIOR;
D O I
10.1016/j.msec.2014.12.011
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
This study aimed to evaluate the cytocompatibility and early osseointegration of Ti-24 Nb-4 Zr-7.9 Sn (Ti-2448) surfaces that were modified with a nanoscale TiO2 coating. The coating was fabricated using a hydrothermal synthesis method to generate nanoTiO(2)/H-2448. The surface characteristics of the samples were evaluated using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS) and X-ray diffraction (XRD). The cytotoxicity of the fabricated nanoTiO(2)/Ti-2448 was determined using MTT assays. The proliferation and alkaline phosphatase (ALP) activity of MC3T3-E1 osteoblasts cultured on nanoTiO(2)/Ti-2448 were compared with those cultured on Ti-2448. Disk-shaped implants were placed in Wistar rats. The histological sections were stained with haematoxylin and eosin (HE), and the histocompatibility was analysed at 4 and 12 weeks post-implantation. Cylindrical implants were embedded in Japanese white rabbits, and the histological sections were stained with HE and anti-TGF-beta 1 to evaluate the histocompatibility and early osseointegration at 4, 12 and 26 weeks post-implantation. NanoTiO(2)/Ti-2448 exhibited a rougher surface than did Ti-2448. NanoTiO(2)/Ti-2448 was determined to be non-cytotoxic. More osteoblasts and higher ALP activity were observed for nanoTiO(2)/Ti-2448 than Ti-2448 < 0.05), Few inflammatory cells were detected around nanoTiO(2)/Ti-2448, and the expression of TGF-beta 1 on nanoTiO(2)/Ti-2448 peaked at earlier time than that on Ti-2448. The results indicate that the cytocompatibility and early osseointegration were enhanced by the nanoTiO(2) coating. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:256 / 262
页数:7
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