A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia

被引：8

作者：

Liu, Zheng ^{[1
]}

Huo, Jin-hai ^{[1
]}

Dong, Wen-ting ^{[1
]}

Sun, Guo-dong ^{[1
]}

Li, Feng-jin ^{[1
]}

Zhang, Ya-nan ^{[1
]}

Qin, Zhi-wei ^{[1
]}

Pengna, Jiang ^{[2
]}

Wang, Wei-ming ^{[1
]}

机构：

[1] Inst Chinese Mat Med, Heilongjiang Acad Chinese Med, Harbin, Peoples R China

[2] Heilongjiang Univ Chinese Med, Harbin, Peoples R China

来源：

FRONTIERS IN PHARMACOLOGY | 2021年 / 12卷

基金：

中国国家自然科学基金;

关键词：

Qinbaiqingfei concentrated pills; metabolomics; network pharmacology; NF-kappa B signaling pathway; mycoplasma pneumonia; COMMUNITY-ACQUIRED PNEUMONIA; MACROLIDE-RESISTANT; PATHOGENESIS; INFECTION; CHILDREN; MANIFESTATIONS; INFLAMMATION; FLAVONOIDS; STRATEGIES; RECEPTORS;

D O I：

10.3389/fphar.2021.761883

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Qinbaiqingfei concentrated pills (QB) are a commonly used medicine for the treatment of mycoplasma pneumonia in China, and the mechanism of action of QB needs to be studied further. Therefore, we use a combination of metabolomics and network pharmacology to clarify the mechanism of QB. Nontarget metabolomics studies were performed on rat serum, urine, and lung tissues, and 56 therapeutic biomarkers were found. Subsequently, the components of QB absorbed into the blood and lung tissues were clarified, and based on this finding, the core target of network pharmacology was predicted. The enrichment analysis of biomarkers-genes finally confirmed their close relationship with the NF-kappa B signaling pathway. By western blotting expression of the proteins in the lung tissue-related signaling pathways, it is finally confirmed that QB inhibits the NF-kappa B signaling pathway through SIRT1, IL-10 and MMP9, CTNNB1, EGFR, and other targets. It plays a role in regulating immunity, regulating metabolism, and treating diseases.

引用

页数：19

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