A REC8-dependent plant shugoshin is required for maintenance of centrorneric cohesion during meiosis and has no mitotic functions

被引:80
作者
Hamant, O
Golubovskaya, I
Meeley, R
Fiume, E
Timofejeva, L
Schleiffer, A
Nasmyth, K
Cande, WZ [1 ]
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Pioneer HiBred Int Inc, Johnston, IA 50131 USA
[3] Res Inst Mol Biol IMP, A-1030 Vienna, Austria
关键词
D O I
10.1016/j.cub.2005.04.049
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
During meiosis, sequential release of sister chromatid cohesion (SSC) during two successive nuclear divisions allows the production of haploid gametes from diploid progenitor cells. Release of SSC along chromosome arms allows first a reductional segregation of homologs, and, subsequently, release of centromeric cohesion at anaphase 11 allows the segregation of chromatids [1-3]. The Shugoshin (SGO) protein family plays a major role in the protection of centromeric cohesion in Drosophila and yeast [4-12]. We have isolated a maize mutant that displays premature loss of centromeric cohesion at anaphase 1. We showed that this phenotype is due to the absence of ZmSGO1 protein, a maize shugoshin homolog. We also show that ZmSGO1 is localized to the centromeres. The ZmSGO1 protein is not found on mitotic chromosomes and has no obvious mitotic function. On the basis of these results, we propose that ZmSGO1 specifically maintains centromeric cohesion during meiosis I and therefore suggest that SGO1 core functions during meiosis are conserved across kingdoms and in large-genome species. However, in contrast to other Shugoshins, we observed an early and REC8-dependent recruitment of ZmSGO1 in maize, suggesting that control of SGO1 recruitment to chromosomes is different in plants than in other model organisms.
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页码:948 / 954
页数:7
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