Comprehensive Analysis of the Mouse Brain Proteome Sampled in Mass Spectrometry Imaging

被引：39

作者：

Heijs, Bram ^{[1
]}

Carreira, Ricardo J. ^{[1
]}

Tolner, Else A. ^{[2
]}

de Ru, Arnoud H. ^{[3
]}

van den Maagdenberg, Arn J. M. ^{[2
,4
]}

van Veelen, Peter A. ^{[3
]}

McDonnell, Liam A. ^{[1
,5
]}

机构：

[1] Leiden Univ, Ctr Prote & Metabol, Med Ctr, NL-2333 ZA Leiden, Netherlands

[2] Leiden Univ, Dept Neurol, Med Ctr, NL-2333 ZA Leiden, Netherlands

[3] Leiden Univ, Dept Immunohematol & Blood Transfus, Med Ctr, NL-2333 ZA Leiden, Netherlands

[4] Leiden Univ, Dept Human Genet, Med Ctr, NL-2333 ZA Leiden, Netherlands

[5] Fdn Pisana Sci ONLUS, I-56121 Pisa, Italy

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 03期

基金：

芬兰科学院;

关键词：

LASER-DESORPTION IONIZATION; SEQUENCE COVERAGE; TISSUE; IDENTIFICATION; PROTEINS; PEPTIDES; DISEASE; CLASSIFICATION; IMPACT;

D O I：

10.1021/ac503952q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On-tissue enzymatic digestion is performed in mass spectrometry imaging (MSI) experiments to access larger proteins and to assign protein identities. Most on-tissue digestion MSI studies have focused on method development rather than identifying the molecular features observed. Herein, we report a comprehensive study of the mouse brain proteome sampled by MSI. Using complementary proteases, we were able to identify 5337 peptides in the matrix-assisted laser desorption/ionization (MALDI) matrix, corresponding to 1198 proteins. 630 of these peptides, corresponding to 280 proteins, could be assigned to peaks in MSI data sets. Gene ontology and pathway analyses revealed that many of the proteins are involved in neurodegenerative disorders, such as Alzheimers, Parkinsons, and Huntingtons disease.

引用

页码：1867 / 1875

页数：9

共 40 条

[31] Elastase Digests NEW AMMUNITION FOR SHOTGUN MEMBRANE PROTEOMICS [J].

Rietschel, Benjamin ;

Arrey, Tabiwang N. ;

Meyer, Bjoern ;

Bornemann, Sandra ;

Schuerken, Malte ;

Karas, Michael ;

Poetsch, Ansgar .

MOLECULAR & CELLULAR PROTEOMICS, 2009, 8 (05) :1029-1043

[32] High-resolution matrix-assisted laser desorption/ionization imaging of tryptic peptides from tissue [J].

Schober, Yvonne ;

Guenther, Sabine ;

Spengler, Bernhard ;

Roempp, Andreas .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2012, 26 (09) :1141-1146

[33] Protein identification by accurate mass matrix-assisted laser desorption/ionization imaging of tryptic peptides [J].

Schober, Yvonne ;

Schramm, Thorsten ;

Spengler, Bernhard ;

Roempp, Andreas .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2011, 25 (17) :2475-2483

[34]

Shuichi S., 2006, SURF INTERFACE ANAL, V38, P1712

[35] Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa [J].

Signor, Luca ;

Erba, Elisabetta Boeri .

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2013, (79)

[36] mMass data miner: an open source alternative for mass spectrometric data analysis [J].

Strohalm, Martin ;

Hassman, Martin ;

Kosata, Bedrich ;

Kodicek, Milan .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2008, 22 (06) :905-908

[37] GETTING INTIMATE WITH TRYPSIN, THE LEADING PROTEASE IN PROTEOMICS [J].

Vandermarliere, Elien ;

Mueller, Michael ;

Martens, Lennart .

MASS SPECTROMETRY REVIEWS, 2013, 32 (06) :453-465

[38] Obtaining high sequence coverage in matrix-assisted laser desorption time-of-flight mass spectrometry for studies of protein modification: Analysis of human serum albumin as a model [J].

Wa, CL ;

Cerny, R ;

Hage, DS .

ANALYTICAL BIOCHEMISTRY, 2006, 349 (02) :229-241

[39]

ZAGON IS, 1986, J NEUROSCI, V6, P2977

[40] Neurovascular pathways to neurodegeneration in Alzheimer's disease and other disorders [J].

Zlokovic, Berislav V. .

NATURE REVIEWS NEUROSCIENCE, 2011, 12 (12) :723-738

← 1 2 3 4 →