Comprehensive Analysis of the Mouse Brain Proteome Sampled in Mass Spectrometry Imaging

被引：39

作者：

Heijs, Bram ^{[1
]}

Carreira, Ricardo J. ^{[1
]}

Tolner, Else A. ^{[2
]}

de Ru, Arnoud H. ^{[3
]}

van den Maagdenberg, Arn J. M. ^{[2
,4
]}

van Veelen, Peter A. ^{[3
]}

McDonnell, Liam A. ^{[1
,5
]}

机构：

[1] Leiden Univ, Ctr Prote & Metabol, Med Ctr, NL-2333 ZA Leiden, Netherlands

[2] Leiden Univ, Dept Neurol, Med Ctr, NL-2333 ZA Leiden, Netherlands

[3] Leiden Univ, Dept Immunohematol & Blood Transfus, Med Ctr, NL-2333 ZA Leiden, Netherlands

[4] Leiden Univ, Dept Human Genet, Med Ctr, NL-2333 ZA Leiden, Netherlands

[5] Fdn Pisana Sci ONLUS, I-56121 Pisa, Italy

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 03期

基金：

芬兰科学院;

关键词：

LASER-DESORPTION IONIZATION; SEQUENCE COVERAGE; TISSUE; IDENTIFICATION; PROTEINS; PEPTIDES; DISEASE; CLASSIFICATION; IMPACT;

D O I：

10.1021/ac503952q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On-tissue enzymatic digestion is performed in mass spectrometry imaging (MSI) experiments to access larger proteins and to assign protein identities. Most on-tissue digestion MSI studies have focused on method development rather than identifying the molecular features observed. Herein, we report a comprehensive study of the mouse brain proteome sampled by MSI. Using complementary proteases, we were able to identify 5337 peptides in the matrix-assisted laser desorption/ionization (MALDI) matrix, corresponding to 1198 proteins. 630 of these peptides, corresponding to 280 proteins, could be assigned to peaks in MSI data sets. Gene ontology and pathway analyses revealed that many of the proteins are involved in neurodegenerative disorders, such as Alzheimers, Parkinsons, and Huntingtons disease.

引用

页码：1867 / 1875

页数：9

共 40 条

[1] The impact of buffers and surfactants from micellar electrokinetic chromatography on matrix-assisted laser desorption ionization (MALDI) mass spectrometry of peptides - Effect of buffer type and concentration on mass determination by MALDI-time-of-flight mass spectrometry [J].

Amini, A ;

Dormady, SJ ;

Riggs, L ;

Regnier, FE .

JOURNAL OF CHROMATOGRAPHY A, 2000, 894 (1-2) :345-355

[2] Classification of HER2/neu Status in Gastric Cancer Using a Breast-Cancer Derived Proteome Classifier [J].

Balluff, Benjamin ;

Elsner, Mareike ;

Kowarsch, Andreas ;

Rauser, Sandra ;

Meding, Stephan ;

Schuhmacher, Christoph ;

Feith, Marcus ;

Herrmann, Ken ;

Rocken, Christoph ;

Schmid, Roland M. ;

Hofler, Heinz ;

Walch, Axel ;

Ebert, Matthias P. .

JOURNAL OF PROTEOME RESEARCH, 2010, 9 (12) :6317-6322

[3] Software Tool for Researching Annotations of Proteins: Open-Source Protein Annotation Software with Data Visualization [J].

Bhatia, Vivek N. ;

Perlman, David H. ;

Costello, Catherine E. ;

McComb, Mark E. .

ANALYTICAL CHEMISTRY, 2009, 81 (23) :9819-9823

[4] The importance of the digest: Proteolysis and absolute quantification in proteomics [J].

Brownridge, Philip ;

Beynon, Robert J. .

METHODS, 2011, 54 (04) :351-360

[5] Molecular imaging of biological samples: Localization of peptides and proteins using MALDI-TOF MS [J].

Caprioli, RM ;

Farmer, TB ;

Gile, J .

ANALYTICAL CHEMISTRY, 1997, 69 (23) :4751-4760

[6] MALDI tissue imaging: from biomarker discovery to clinical applications [J].

Cazares, Lisa H. ;

Troyer, Dean A. ;

Wang, Binghe ;

Drake, Richard R. ;

Semmes, O. John .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 401 (01) :17-27

[7] Multiple enzymatic digestion for enhanced sequence coverage of proteins in complex proteomic mixtures using capillary LC with ion trap MS/MS [J].

Choudhary, G ;

Wu, SL ;

Shieh, P ;

Hancock, WS .

JOURNAL OF PROTEOME RESEARCH, 2003, 2 (01) :59-67

[8] MALDI-Ion Mobility Separation-Mass Spectrometry Imaging of Glucose-Regulated Protein 78 kDa (Grp78) in Human Formalin-Fixed, Paraffin-Embedded Pancreatic Adenocarcinoma Tissue Sections [J].

Djidja, Marie-Claude ;

Claude, Emmanuelle ;

Snel, Marten F. ;

Scriven, Peter ;

Francese, Simona ;

Carolan, Vikki ;

Clench, Malcolm R. .

JOURNAL OF PROTEOME RESEARCH, 2009, 8 (10) :4876-4884

[9] Detergent addition to tryptic digests and ion mobility separation prior to MS/MS improves peptide yield and protein identification for in situ proteomic investigation of frozen and formalin-fixed paraffin-embedded adenocarcinoma tissue sections [J].

Djidja, Marie-Claude ;

Francese, Simona ;

Loadman, Paul M. ;

Sutton, Chris W. ;

Scriven, Peter ;

Claude, Emmanuelle ;

Snel, Marten F. ;

Franck, Julien ;

Salzet, Michel ;

Clench, Malcolm R. .

PROTEOMICS, 2009, 9 (10) :2750-2763

[10] Influence of various on-tissue washing procedures on the entire protein quantity and the quality of matrix-assisted laser desorption/ionization spectra [J].

Enthaler, Bernd ;

Bussmann, Tanja ;

Pruns, Julia K. ;

Rapp, Claudius ;

Fischer, Markus ;

Vietzke, Jens-Peter .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2013, 27 (08) :878-884

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