Novel Evolved Immunoglobulin (Ig)-Binding Molecules Enhance the Detection of IgM against Hepatitis C Virus

被引:16
作者
Cao, Jie [1 ]
Chen, Qiuli [1 ]
Zhang, Huaqun [1 ]
Qi, Peipei [1 ]
Liu, Chao [1 ]
Yang, Xufang [1 ]
Wang, Niansong [2 ,3 ]
Qian, Baohua [4 ]
Wang, Jinhong [1 ]
Jiang, Shaohua [1 ,5 ]
Yang, Hua [1 ,6 ]
Sun, Shuhan [7 ]
Pan, Wei [1 ]
机构
[1] Second Mil Med Univ, Shanghai Key Lab Med Biodef, Dept Microbiol, Shanghai, Peoples R China
[2] Fudan Univ, Zhongshan Hosp, Dept Nephrol, Shanghai 200433, Peoples R China
[3] Shanghai Jiao Tong Univ, Dept Nephrol, Shanghai Peoples Hosp 6, Shanghai 200030, Peoples R China
[4] Second Mil Med Univ, Changhai Hosp, Dept Blood Transfus, Shanghai, Peoples R China
[5] Mil Gen Hosp Tibet PLA, Dept Cardiothorac Surg, Lasa, Peoples R China
[6] Tongji Univ, Sch Med, Shanghai Pulm Hosp, Shanghai Key Lab TB, Shanghai 200092, Peoples R China
[7] Second Mil Med Univ, Dept Med Genet, Shanghai, Peoples R China
基金
中国国家自然科学基金;
关键词
POLYMERASE-CHAIN-REACTION; B-CELL ACTIVATION; ANTI-HCV; CLINICAL-COURSE; NON-A; 2ND-GENERATION; HEMODIALYSIS; ANTIBODIES; INFECTION; BINDING;
D O I
10.1371/journal.pone.0018477
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Detection of specific antibodies against hepatitis C virus (HCV) is the most widely available test for viral diagnosis and monitoring of HCV infections. However, narrowing the serologic window of anti-HCV detection by enhancing anti-HCV IgM detection has remained to be a problem. Herein, we used LD5, a novel evolved immunoglobulin-binding molecule (NEIBM) with a high affinity for IgM, to develop a new anti-HCV enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase-labeled LD5 (HRP-LD5) as the conjugated enzyme complex. The HRP-LD5 assay showed detection efficacy that is comparable with two kinds of domestic diagnostic kits and the Abbott 3.0 kit when tested against the national reference panel. Moreover, the HRP-LD5 assay showed a higher detection rate (55.9%, 95% confidence intervals (95% CI, 0.489, 0.629) than that of a domestic diagnostic ELISA kit (Chang Zheng) (53.3%, 95% CI 0.463, 0.603) in 195 hemodialysis patient serum samples. Five serum samples that were positive using the HRP-LD5 assay and negative with the conventional anti-HCV diagnostic ELISA kits were all positive for HCV RNA, and 4 of them had detectable antibodies when tested with the established anti-HCV IgM assay. An IgM confirmation study revealed the IgM reaction nature of these five serum samples. These results demonstrate that HRP-LD5 improved anti-HCV detection by enhancing the detection of anti-HCV IgM, which may have potential value for the early diagnosis and screening of hepatitis C and other infectious diseases.
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页数:7
相关论文
共 35 条
[1]   HEPATITIS-C VIRUS-INFECTION IN POSTTRANSFUSION HEPATITIS - AN ANALYSIS WITH 1ST-GENERATION AND 2ND-GENERATION ASSAYS [J].
AACH, RD ;
STEVENS, CE ;
HOLLINGER, FB ;
MOSLEY, JW ;
PETERSON, DA ;
TAYLOR, PE ;
JOHNSON, RG ;
BARBOSA, LH ;
NEMO, GJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1991, 325 (19) :1325-1329
[2]   Comparison of second- and third-generation enzyme Immunoassays for detecting antibodies to hepatitis C virus [J].
Abdel-Hamid, M ;
El-Daly, M ;
El-Kafrawy, S ;
Mikhail, N ;
Strickland, GT ;
Fix, AD .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (05) :1656-1659
[3]   ANTI-HCV IMMUNOGLOBULIN-M ANTIBODY IN PATIENTS WITH ACUTE AND FULMINANT HEPATITIS-C [J].
AKBAR, SMF ;
ONJI, M ;
HORIIKE, N ;
OHTA, Y .
GASTROENTEROLOGIA JAPONICA, 1993, 28 :71-75
[4]  
Al-Faleh F Z, 1995, Saudi J Kidney Dis Transpl, V6, P118
[6]  
[Anonymous], 1997, HEPATOLOGY, V26, pS2
[7]   HCV VIREMIA IN HEMODIALYSIS-PATIENTS - DETECTION BY A DNA ENZYME-IMMUNOASSAY FOR AMPLIFIED HCV SEQUENCES [J].
BOERO, R ;
MARTINA, G ;
BOSIO, P ;
DEVOS, S ;
BERTOLO, P ;
FORNERIS, G ;
GIACHINO, O ;
QUARELLO, F ;
PICCOLI, G .
RENAL FAILURE, 1995, 17 (05) :565-573
[8]   Sensitive detection of human IgG in ELISA using a monoclonal anti-IgG-peroxidase conjugate [J].
Chevrier, MC ;
Châteauneuf, I ;
Guérin, M ;
Lemieux, R .
HYBRIDOMA AND HYBRIDOMICS, 2004, 23 (06) :362-367
[9]   A LONG-TERM STUDY OF HEPATITIS-C VIRUS-REPLICATION IN NON-A, NON-B HEPATITIS [J].
FARCI, P ;
ALTER, HJ ;
WONG, D ;
MILLER, RH ;
SHIH, JW ;
JETT, B ;
PURCELL, RH .
NEW ENGLAND JOURNAL OF MEDICINE, 1991, 325 (02) :98-104
[10]   DETECTION OF HEPATITIS-C VIRAL SEQUENCES IN BLOOD DONATIONS BY NESTED POLYMERASE CHAIN-REACTION AND PREDICTION OF INFECTIVITY [J].
GARSON, JA ;
TEDDER, RS ;
BRIGGS, M ;
TUKE, P ;
GLAZEBROOK, JA ;
TRUTE, A ;
PARKER, D ;
BARBARA, JAJ ;
CONTRERAS, M ;
ALOYSIUS, S .
LANCET, 1990, 335 (8703) :1419-1422