Physiological Degradation of Pectin in Papaya Cell Walls: Release of Long Chains Galacturonans Derived from Insoluble Fractions during Postharvest Fruit Ripening

被引:34
作者
do Prado, Samira B. R. [1 ]
Melfi, Paulo R. [1 ]
Castro-Alves, Victor C. [1 ]
Broetto, Sabrina G. [1 ]
Araujo, Elias S. [1 ]
do Nascimento, Joao R. O. [1 ,2 ,3 ]
Fabi, Joao P. [1 ,2 ,3 ]
机构
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo, Brazil
[2] Univ Sao Paulo, NAPAN Food & Nutr Res Ctr, Sao Paulo, Brazil
[3] Sao Paulo Res Fdn, CEPID FAPESP Res Innovat & Disseminat Ctr, Food Res Ctr, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
papaya ripening; galacturonan; cell wall disassembly; pectin depolymerization; fruit softening; CELLULOSE; XYLOGLUCAN; EXPRESSION; ETHYLENE; CLONING; MODELS; PLANTS;
D O I
10.3389/fpls.2016.01120
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Papaya (Car/ca papaya L.) is a fleshy fruit that presents a rapid pulp softening during ripening. However, the timeline on how papaya pectinases act in polysaccharide solubilization and the consequent modification of the cell wall fractions during ripening is still not clear. In this work, the gene expression correlations between, on one hand, 16 enzymes potentially acting during papaya cell wall disassembling and, on the other hand, the monosaccharide composition of cell wall fractions during papaya ripening were evaluated. In order to explain differences in the ripening of papaya samplings, the molecular mass distribution of polysaccharides from water-soluble and oxalate -soluble fractions NSF and OSF, respectively), as well as the oligosaccharide profiling from the WSF fraction, were evaluated by high performance size exclusion chromatography coupled to a refractive index detector and high performance anion -exchange chromatography coupled to pulse amperometric detection analyses, respectively. Results showed that up -regulated polygalacturonase and f)-galactosidase genes were positively correlated with some monosaccharide profiles. In addition, an overall increase in the retention time of high molecular weight (HMW) and low molecular weight (LMVV) polysaccharides in VVSF and OSF was shown. The apparent disappearance of one HMVV peak of the OSF may result from the conversion of pectin that were crosslinked with calcium into more soluble forms through the action of PGs, which would increase the solubilization of polysaccharides by lowering their molecular weight. Thus, the results allowed us to propose a detailed process of papaya cell wall disassembling that would affect sensorial properties and post -harvesting losses of this commercially important fruit.
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页数:11
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共 33 条
[1]   Biochemical and physiological study of the firmness of table grape berries [J].
Balic, Ivan ;
Ejsmentewicz, Troy ;
Sanhueza, Dayan ;
Silva, Christian ;
Peredo, Tamara ;
Olmedo, Patricio ;
Barros, Miriam ;
Verdonk, Julian C. ;
Paredes, Rodolfo ;
Meneses, Claudio ;
Prieto, Humberto ;
Orellana, Ariel ;
Defilippi, Bruno G. ;
Campos-Vargas, Reinaldo .
POSTHARVEST BIOLOGY AND TECHNOLOGY, 2014, 93 :15-23
[2]   Cloning and expression analysis of two putative papaya genes encoding polygalacturonase-inhibiting proteins [J].
Broetto, Sabrina Garcia ;
Fabi, Joao Paulo ;
do Nascimento, Joao-Roberto Oliveira .
POSTHARVEST BIOLOGY AND TECHNOLOGY, 2015, 104 :48-56
[3]   Cell wall disassembly in ripening fruit [J].
Brummell, DA .
FUNCTIONAL PLANT BIOLOGY, 2006, 33 (02) :103-119
[4]   Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants [J].
Brummell, DA ;
Harpster, MH .
PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) :311-340
[5]   The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].
Bustin, Stephen A. ;
Benes, Vladimir ;
Garson, Jeremy A. ;
Hellemans, Jan ;
Huggett, Jim ;
Kubista, Mikael ;
Mueller, Reinhold ;
Nolan, Tania ;
Pfaffl, Michael W. ;
Shipley, Gregory L. ;
Vandesompele, Jo ;
Wittwer, Carl T. .
CLINICAL CHEMISTRY, 2009, 55 (04) :611-622
[6]   STRUCTURAL MODELS OF PRIMARY-CELL WALLS IN FLOWERING PLANTS - CONSISTENCY OF MOLECULAR-STRUCTURE WITH THE PHYSICAL-PROPERTIES OF THE WALLS DURING GROWTH [J].
CARPITA, NC ;
GIBEAUT, DM .
PLANT JOURNAL, 1993, 3 (01) :1-30
[7]   Re-constructing our models of cellulose and primary cell wall assembly [J].
Cosgrove, Daniel J. .
CURRENT OPINION IN PLANT BIOLOGY, 2014, 22 :122-131
[8]   The self-assembled network and physiological degradation of pectins in carrot cell walls [J].
Cybulska, Justyna ;
Zdunek, Artur ;
Kozio, Arkadiusz .
FOOD HYDROCOLLOIDS, 2015, 43 :41-50
[9]   How to let go: pectin and plant cell adhesion [J].
Daher, Firas Bou ;
Braybrook, Siobhan A. .
FRONTIERS IN PLANT SCIENCE, 2015, 6
[10]   Papaya fruit ripening: Response to ethylene and 1-methylcyclopropene (1-MCP) [J].
Fabi, Joao Paulo ;
Cordenunsi, Beatriz Rosana ;
De Mattos Barreto, Gisela P. ;
Mercadante, Adriana Z. ;
Lajolo, Franco M. ;
Do Nascimento, Joao Roberto Oliveira .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (15) :6118-6123