Bioactivity-guided fractionation for anti-inflammatory and analgesic properties and constituents of Xanthium strumarium L.

被引：78

作者：

Han, T.

Li, H. -L.

Zhang, Q. -Y.

Han, P.

Zheng, H. -C.

Rahman, K.

Qin, L. -P. ^{[1
]}

机构：

[1] Mil Med Univ 2, Sch Pharm, Dept Pharmacognosy, Shanghai 200433, Peoples R China

[2] Mil Med Univ 2, Sch Pharm, Dept Nat Med Chem, Shanghai 200433, Peoples R China

[3] Liverpool John Moores Univ, Sch Biomol Sci, Fac Sci, Liverpool L3 3AF, Merseyside, England

来源：

PHYTOMEDICINE | 2007年 / 14卷 / 12期

关键词：

Xanthium strumarium; anti-inflammatory; analgesic; croton-oil-induced ear edema; acetic-acid-induced writhing; bioassay-guided isolation;

D O I：

10.1016/j.phymed.2007.01.010

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The aim of this study was to fractionate an extract of Xanthium strumarium L. (EXS) and to investigate the antiinflammatory and analgesic properties of the extract and its fractions. The ethanol extract of X. strumarium (EXS) was fractionated on the basis of polarity. Among the different fractions, the n-butanol fraction showed the highest antiinflammatory activity in the croton-oil-induced ear edema test and furthermore reduced the number of writhings induced by acetic acid in mice in a dose-dependent manner. This indicates that the n-butanol fraction of X strumarium possesses potent analgesic effects which are likely to be mediated by its anti-inflammatory activity. Bioassay-guided fractionation of EXS led to the isolation and identification of ten caffeoylquinic acids and three heterocyclics by HPLC-DAD-MSn from the active n-butanol fraction, implying that the active compounds are polar in nature. The isolated caffeoylquinic acids could partially explain the antinociceptive effect of X. strumarium polar extract. (c) 2007 Elsevier GmbH. All rights reserved.

引用

页码：825 / 829

页数：5

共 18 条

[1]

CHEN YL, 1979, FLORA REIPUBLICAE PO, V75, P324

[2] Discriminating between the six isomers of dicaffeoylquinic acid by LC-MSn [J].