Structural characterization and immune-enhancing activity of a novel high-molecular-weight polysaccharide from Cordyceps militaris

被引：79

作者：

He, Bao-Lin ^{[1
,2
,3
]}

Zheng, Qian-Wang ^{[1
,2
,3
]}

Guo, Li-Qiong ^{[1
,2
,3
]}

Huang, Jen-Yi ^{[4
]}

Yun, Fan ^{[5
]}

Huang, Shi-Shi ^{[1
,2
,3
]}

Lin, Jun-Fang ^{[1
,2
,3
]}

机构：

[1] South China Agr Univ, Coll Food Sci, Guangzhou 510640, Peoples R China

[2] South China Agr Univ, Inst Food Biotechnol, Guangzhou 510640, Peoples R China

[3] Res Ctr Microecol Agent Engn & Technol Guangdong, Guangzhou 510640, Peoples R China

[4] Purdue Univ, Dept Food Sci, 745 Agr Mall Dr, W Lafayette, IN 47907 USA

[5] Guangzhou Alchemy Biotechnol Co Ltd, 139 Hongming Rd, Guangzhou 510760, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 145卷

基金：

中国国家自然科学基金;

关键词：

Cordyceps militaris; High-molecular-weight polysaccharide; Structural characterization; Immune-enhancing activity; IMMUNOSTIMULATORY ACTIVITY; IMMUNOMODULATORY ACTIVITY; ANTIOXIDANT ACTIVITIES; FRUITING BODIES; ELUCIDATION; PURIFICATION; MACROPHAGES; EXTRACTION; MYCELIUM;

D O I：

10.1016/j.ijbiomac.2019.12.115

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel homogeneous polysaccharide (CMP-III) was extracted and purified from C. militaris. Structural characterization revealed that CMP-III had an average molecular weight of 4.796 x 10(4) kDa and consisted of glucose, mannose and galactose with the molar ratio of 8.09:1.00:0.25. The main linkage types of CMP-III consisted of 1 -> 4)-alpha-D-Glc (70.08%), 1 -> 4,6)-alpha-D-Man (9.59%), 1 ->)-alpha-D-Man (10.79%) and 1 -> 2,6)-alpha-D-Gal (3.93%) based on methylation and NMR analysis. The immunomodulatory assay indicated that CMP-III significantly promoted macrophage phagocytosis and secretion of NO, TNF-alpha and IL-6. Further study suggested that macrophage activated by CMP-III involved mitogen-activated protein kinases (MAPKs) and nuclear factor kappa-B (NF-kappa B) signaling pathways. Overall, these results suggested that CMP-III could be developed as a potent immunomodulatory agent for use in functional foods and dietary supplements. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：11 / 20

页数：10

共 41 条

[41] Structure and anti-tumor activity of a high-molecular-weight polysaccharide from cultured mycelium of Cordyceps gunnii [J].

Zhu, Zhen-yuan ;

Liu, Nian ;

Si, Chuan-ling ;

Liu, Yang ;

Ding, Li-na ;

Jing, Chen ;

Liu, An-jun ;

Zhang, Yong-min .

CARBOHYDRATE POLYMERS, 2012, 88 (03) :1072-1076

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