Structural characterization and immune-enhancing activity of a novel high-molecular-weight polysaccharide from Cordyceps militaris

被引：79
作者：
He, Bao-Lin [1 ,2 ,3 ]
Zheng, Qian-Wang [1 ,2 ,3 ]
Guo, Li-Qiong [1 ,2 ,3 ]
Huang, Jen-Yi [4 ]
Yun, Fan [5 ]
Huang, Shi-Shi [1 ,2 ,3 ]
Lin, Jun-Fang [1 ,2 ,3 ]
机构：
[1] South China Agr Univ, Coll Food Sci, Guangzhou 510640, Peoples R China
[2] South China Agr Univ, Inst Food Biotechnol, Guangzhou 510640, Peoples R China
[3] Res Ctr Microecol Agent Engn & Technol Guangdong, Guangzhou 510640, Peoples R China
[4] Purdue Univ, Dept Food Sci, 745 Agr Mall Dr, W Lafayette, IN 47907 USA
[5] Guangzhou Alchemy Biotechnol Co Ltd, 139 Hongming Rd, Guangzhou 510760, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 145卷
基金：
中国国家自然科学基金;
关键词：
Cordyceps militaris; High-molecular-weight polysaccharide; Structural characterization; Immune-enhancing activity; IMMUNOSTIMULATORY ACTIVITY; IMMUNOMODULATORY ACTIVITY; ANTIOXIDANT ACTIVITIES; FRUITING BODIES; ELUCIDATION; PURIFICATION; MACROPHAGES; EXTRACTION; MYCELIUM;
D O I：
10.1016/j.ijbiomac.2019.12.115
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel homogeneous polysaccharide (CMP-III) was extracted and purified from C. militaris. Structural characterization revealed that CMP-III had an average molecular weight of 4.796 x 10(4) kDa and consisted of glucose, mannose and galactose with the molar ratio of 8.09:1.00:0.25. The main linkage types of CMP-III consisted of 1 -> 4)-alpha-D-Glc (70.08%), 1 -> 4,6)-alpha-D-Man (9.59%), 1 ->)-alpha-D-Man (10.79%) and 1 -> 2,6)-alpha-D-Gal (3.93%) based on methylation and NMR analysis. The immunomodulatory assay indicated that CMP-III significantly promoted macrophage phagocytosis and secretion of NO, TNF-alpha and IL-6. Further study suggested that macrophage activated by CMP-III involved mitogen-activated protein kinases (MAPKs) and nuclear factor kappa-B (NF-kappa B) signaling pathways. Overall, these results suggested that CMP-III could be developed as a potent immunomodulatory agent for use in functional foods and dietary supplements. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码：11 / 20
页数：10
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