Characterization of Collagen from Different Discarded Fish Species of the West Coast of the Iberian Peninsula

被引:72
作者
Sotelo, Carmen G. [1 ]
Blanco Comesana, Maria [1 ]
Ramos Ariza, Patricia [1 ]
Perez-Martin, Ricardo I. [1 ]
机构
[1] CSIC, Inst Invest Marinas, Eduardo Cabello 6, Vigo 36208, Spain
关键词
Fish skin; discards; collagen; hydroxyproline; I COLLAGEN; SKIN; IDENTIFICATION; PROPERTY; SCALE; ACID;
D O I
10.1080/10498850.2013.865283
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Skin collagen of six discarded fish species was analyzed and compared. Acid soluble collagen (ASC) was extracted; a characteristic sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) profile for type I collagen was obtained, except for Chimaera mostrosa. Contents of collagen calculated from HPro (31.85% average) were higher than those determined from ASC extracts (17.75% average), with Galeus spp. being the species with the higher percentage. Amino acid analysis revealed the typical composition of collagen, with very few differences among species. Specific profiles were obtained after protease digestion. Denaturation temperature of ASC correlated well with imino and hydroxyproline contents.Results demonstrate the feasibility of using the obtained collagens in different industrial applications.
引用
收藏
页码:388 / 399
页数:12
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