Repair of spinal cord injury by neural stem cells transfected with brain-derived neurotrophic factor-green fluorescent protein in rats A double effect of stem cells and growth factors?

被引：3
作者：
Wang, Yansong [1 ]
Lue, Gang [1 ]
机构：
[1] China Med Univ, Affiliated Hosp 1, Dept Orthoped, Shenyang 110001, Liaoning, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2010年 / 5卷 / 17期
关键词：
neural stem cells; brain-derived neurotrophic factor; transplantation; green fluorescent protein; spinal cord injury; neural regeneration; FUNCTIONAL RECOVERY; CORTICOSPINAL NEURONS; PROGENITOR CELLS; ADULT RATS; LONG-TERM; TRANSPLANTATION; EXPRESSION; BDNF; NGF; DIFFERENTIATION;
D O I：
10.3969/j.issn.1673-5374.2010.17.004
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Brain-derived neurotrophic factor (BDNF) can significantly promote nerve regeneration and repair. High expression of the BDNF-green fluorescent protein (GFP) gene persists for a long time after transfection into neural stem cells. Nevertheless, little is known about the biological characteristics of BDNF-GFP modified nerve stem cells in vivo and their ability to induce BDNF expression or repair spinal cord injury. In the present study, we transplanted BDNF-GFP transgenic neural stem cells into a hemisection model of rats. Rats with BDNF-GFP stem cells exhibited significantly increased BDNF expression and better locomotor function compared with stem cells alone. Cellular therapy with BDNF-GFP transgenic stem cells can improve outcomes better than stem cells alone and may have therapeutic potential for spinal cord injury.
引用
收藏
页码：1303 / 1307
页数：5
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