Cucurbit aphid-borne yellows virus from melon plants in Brazil is an interspecific recombinant

被引:14
作者
Costa, Thiago Marques [1 ]
Blawid, Rosana [1 ]
Aranda, Miguel A. [2 ]
Sansini Freitas, Debora Maria [3 ]
Andrade, Genira Pereira [4 ]
Inoue-Nagata, Alice Kazuko [5 ]
Nagata, Tatsuya [1 ]
机构
[1] Univ Brasilia, Dept Biol Celular, Campus Darcy Ribeiro, BR-70910900 Brasilia, DF, Brazil
[2] CSIC, CEBAS, Murcia 30100, Spain
[3] Embrapa Semiarido, BR-56302970 Petrolina, PE, Brazil
[4] Univ Fed Rural Pernambuco, Dept Agron, BR-52171900 Recife, PE, Brazil
[5] Embrapa Hortalicas, BR-70275970 Brasilia, DF, Brazil
关键词
COMPLETE GENOME SEQUENCE; DISEASE; MODELS; CROPS;
D O I
10.1007/s00705-018-4024-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Melon plants with severe yellowing symptoms from in Brazil were analyzed by high-throughput sequencing. Sequences homologous to the genome of the polerovirus cucurbit aphid-borne yellows virus (CABYV) were frequently retrieved. Two draft CABYV genomes were assembled from two pooled melon samples that contained an identical putative recombinant fragment in the 3 region with an unknown polerovirus. The complete genomes of these isolates revealed by Sanger sequencing share 96.8% nucleotide identity, while both sequences share 73.7% nucleotide identity with a CABYV-N isolate from France. A molecular-clock analysis suggested that CABYV was introduced into Brazil similar to 68years ago.
引用
收藏
页码:249 / 254
页数:6
相关论文
共 25 条
[1]   SPREAD: spatial phylogenetic reconstruction of evolutionary dynamics [J].
Bielejec, Filip ;
Rambaut, Andrew ;
Suchard, Marc A. ;
Lemey, Philippe .
BIOINFORMATICS, 2011, 27 (20) :2910-2912
[2]   Discovering and sequencing new plant viral genomes by next-generation sequencing: description of a practical pipeline [J].
Blawid, R. ;
Silva, J. M. F. ;
Nagata, T. .
ANNALS OF APPLIED BIOLOGY, 2017, 170 (03) :301-314
[3]   Complete genome sequence of melon yellowing-associated virus from melon plants with the severe yellowing disease in Brazil [J].
Costa, Thiago M. ;
Blawid, Rosana ;
da Costa Junior, Avanor C. ;
Lima, Mirtes F. ;
de Aragao, Fernando A. S. ;
de Andrade, Genira P. ;
Pio-Ribeiro, Gilvan ;
Aranda, Miguel A. ;
Inoue-Nagata, Alice K. ;
Nagata, Tatsuya .
ARCHIVES OF VIROLOGY, 2017, 162 (12) :3899-3901
[4]   Polerovirus protein P0 prevents the assembly of small RNA-containing RISC complexes and leads to degradation of ARGONAUTE1 [J].
Csorba, Tibor ;
Lozsa, Rita ;
Hutvagner, Gyoergy ;
Burgyan, Jozsef .
PLANT JOURNAL, 2010, 62 (03) :463-472
[5]   jModelTest 2: more models, new heuristics and parallel computing [J].
Darriba, Diego ;
Taboada, Guillermo L. ;
Doallo, Ramon ;
Posada, David .
NATURE METHODS, 2012, 9 (08) :772-772
[6]   ProtTest 3: fast selection of best-fit models of protein evolution [J].
Darriba, Diego ;
Taboada, Guillermo L. ;
Doallo, Ramon ;
Posada, David .
BIOINFORMATICS, 2011, 27 (08) :1164-1165
[7]  
Santos Antonio Apoliano dos, 2008, Summa phytopathol., V34, P359, DOI 10.1590/S0100-54052008000400011
[8]   Bayesian Phylogenetics with BEAUti and the BEAST 1.7 [J].
Drummond, Alexei J. ;
Suchard, Marc A. ;
Xie, Dong ;
Rambaut, Andrew .
MOLECULAR BIOLOGY AND EVOLUTION, 2012, 29 (08) :1969-1973
[9]   NUCLEOTIDE-SEQUENCE OF CUCURBIT APHID-BORNE YELLOWS LUTEOVIRUS [J].
GUILLEY, H ;
WIPFSCHEIBEL, C ;
RICHARDS, K ;
LECOQ, H ;
JONARD, G .
VIROLOGY, 1994, 202 (02) :1012-1017
[10]   A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood [J].
Guindon, S ;
Gascuel, O .
SYSTEMATIC BIOLOGY, 2003, 52 (05) :696-704