NMR spectroscopic structural characterization of a water-soluble β-(1 → 3, 1 → 6)-glucan from Aureobasidium pullulans

被引：53

作者：

Kono, Hiroyuki ^{[1
]}

Kondo, Nobuhiro ^{[2
]}

Hirabayashi, Katsuki ^{[2
]}

Ogata, Makoto ^{[3
]}

Totani, Kazuhide ^{[4
]}

Ikematsu, Shinya ^{[5
]}

Osada, Mitsumasa ^{[6
]}

机构：

[1] Tomakomai Coll, Natl Inst Technol, Div Appl Chem & Biochem, Nishikioka 443, Tomakomai, Hokkaido 0591275, Japan

[2] Itochu Sugar Co Ltd, Tamatsuura 3, Hekinan, Aichi 4478506, Japan

[3] Fukushima Coll, Natl Inst Technol, Dept Chem & Biochem, Nagao 30, Iwaki, Fukushima 9708034, Japan

[4] Ichinoseki Coll, Natl Inst Technol, Dept Engn Future Innovat, Div Chem Engn & Biotechnol, Ichinoseki, Iwate 0218511, Japan

[5] Okinawa Coll, Natl Inst Technol, Dept Bioresources Engn, Henoko 905, Nago, Okinawa 9052192, Japan

[6] Shinshu Univ, Fac Text Sci & Technol, Dept Chem & Mat, 3-15-1 Tokida, Ueda, Nagano 3868567, Japan

来源：

CARBOHYDRATE POLYMERS | 2017年 / 174卷

关键词：

beta-(1; 3; 1; 6)-Glucann; NMR; Schizophyllan; Aureobasidium pullulans; Polysaccharide-polynucleotide complex; POLYSACCHARIDE-POLYNUCLEOTIDE COMPLEXES; SCHIZOPHYLLUM-COMMUNE POLYSACCHARIDE; SINGLE-STRANDED RNA; STRAIN BLACK YEAST; NATURAL POLYSACCHARIDE; GENE DELIVERY; TRIPLE-HELIX; BETA-1,3/1,6-GLUCAN; ANTITUMOR; BEHAVIOR;

D O I：

10.1016/j.carbpol.2017.07.018

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An unambiguous structural characterization of the water-soluble Aureobasidium pullulans beta-(1 -> 3, 1 -> 6)-glucan is yet to be achieved, although this beta-(1 -> 3,1 -> 6)-glucan is expected to exhibit excellent biofunctional properties. Thus, we herein report the elucidation of the primary structure of the A. pullulans beta-(1 -> 3,1 -> 6)-glucan using nuclear magnetic resonance spectroscopy, followed by comparison of the obtained structure with that of schizophyllan (SPG). Structural characterization of the A. pullulans beta-(1 -> 3,1 -> 6)-glucan revealed that the structural units are a beta-(1 -> 3,)-D-glucan backbone with four beta-(1 -> 6)-D-glucosyl side branching units every six residues. In addition, circular dichroism spectroscopic analysis revealed that the beta-(1 -> 3,1 -> 6)-glucan interacted with polyadenylic acid (poly(A)) chains in DMSO solution to form a complex similar to that obtained in the complexation of SPG/poly(A). This finding indicates that beta-(1 > 3,1 > 6)-glucan forms a triple-helical conformation in aqueous solution but exhibits a random coil structure in DMSO solution, which is similar to the behavior of SPG. (C) 2017 Elsevier Ltd. All rights reserved

引用

页码：876 / 886

页数：11

共 40 条

[1] PYTHIUM-APHANIDERMATUM - CULTURE, CELL-WALL COMPOSITION, AND ISOLATION AND STRUCTURE OF ANTITUMOR STORAGE AND SOLUBILIZED CELL-WALL (1 -] 3), (1 -] 6)-BETA-D-GLUCANS [J].