Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity

被引:53
作者
Atiroglu, Vesen [1 ]
机构
[1] Sakarya Univ, Biomed Magnet & Semicond Mat Applicat & Res Ctr B, TR-54187 Sakarya, Turkey
关键词
Immobilization; Graphene oxide; Lipase; Hyaluronic acid; Magnetic nanoparticles; ENZYME AGGREGATES CLEAS; PROTEIN; HYDROLYSIS; DESIGN; PH;
D O I
10.1016/j.ijbiomac.2019.12.233
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids. The recovery of soluble lipase is not possible which makes this assay method expensive. Methods: The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs). Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities. Different techniques were used to analyze the preparation compounds. Results: The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 hat 4 degrees C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%. Storage stability significantly developed for the lipase-GO-MNPs-CLEAs comparing to the free lipase. Conclusions: The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:456 / 465
页数:10
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