Protective Effects of Compounds from Cimicifuga dahurica against Amyloid Beta Production in Vitro and Scopolamine-Induced Memory Impairment in Vivo

被引：11

作者：

Lee, Sang-Bin ^{[3
,5
]}

Yang, Seo Young ^{[2
]}

Nguyen Phuong Thao ^{[6
]}

Seo, Dae-Gun ^{[1
]}

Kim, Sunggun ^{[1
]}

Chi Thanh Ma ^{[3
]}

Park, So-Young ^{[1
]}

Kim, Young Ho ^{[2
]}

Yang, Hyun Ok ^{[3
,4
]}

机构：

[1] Dankook Univ, Coll Pharm, Lab Pharmacognosy, Cheonan 31116, South Korea

[2] Chungnam Natl Univ, Coll Pharm, Daejeon 34134, South Korea

[3] Korea Inst Sci & Technol, Nat Prod Res Ctr, Kangnung 25451, Gangwon Do, South Korea

[4] Korea Univ Sci & Technol, KIST Sch, Div Biomed Sci & Technol, Seoul 02792, South Korea

[5] Sungkyunkwan Univ, Sch Pharm, Suwon 16419, South Korea

[6] VAST, Inst Marine Biochem IMBC, Hanoi, Vietnam

来源：

JOURNAL OF NATURAL PRODUCTS | 2020年 / 83卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

ALZHEIMERS-DISEASE; DERIVATIVES; ROOTS; CREB; L;

D O I：

10.1021/acs.jnatprod.9b00543

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cimicifuga dahurica has traditionally been used as an antipyretic, analgesic, and anti-inflammatory agent and as a treatment for uterine and anal prolapse. This study has investigated the potential beneficial effects of this medicinal plant and its components on Alzheimer's disease (AD) with a focus on amyloid beta (A beta) production and scopolamine-induced memory impairment in mice. An ethanol extract from C. dahurica roots decreased A beta production in APP-CHO cells [Chinese hamster ovarian (CHO) cells stably expressing amyloid precursor protein (APP)], as determined by an enzyme-linked immunosorbent assay and Western blot analysis. Then, the compounds isolated from C. dahurica were tested for their antiamyloidogenic activities. Four compounds (1-4) efficiently interrupted A beta generation by suppressing the level of beta-secretase in APP-CHO cells. Moreover, the in vivo experimental results demonstrated that compound 4 improved the cognitive performances of mice with scopolamine-induced disruption on behavioral tests and the expression of memory-related proteins. Taken together, these results suggest that C. dahurica and its constituents are potential agents for preventing or alleviating the symptoms of AD.

引用

页码：223 / 230

页数：8

共 28 条

[1] Choline reverses scopolamine-induced memory impairment by improving memory reconsolidation [J].