Ultra-fast gradient vs. fast isocratic chromatography in bioanalytical quantification by liquid chromatography/tandem mass spectrometry

被引：42

作者：

Romanyshyn, L ^{[1
]}

Tiller, PR ^{[1
]}

Alvaro, P ^{[1
]}

Pereira, A ^{[1
]}

Hop, CECA ^{[1
]}

机构：

[1] Merck Res Labs, Drug Metab, Rahway, NJ 07065 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2001年 / 15卷 / 05期

关键词：

D O I：

10.1002/rcm.229

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Liquid chromatagraphy/tandem mass spectrometry (LC/MS/MS) methods developed for quantification using rapid ('ballistic') gradients on narrow bore, short HPLC columns have been previously described by this laboratory. This paper compares the fast gradient approach with the more traditional high-organic isocratic LC/MS/MS methods. The comparison is based on an analysis of the effectiveness of the chromatographic separations when using the two approaches (i.e. k' N, and W). The data presented herein are derived from actual biological samples analyzed as part of the drug discovery process. Copyright (C) 2001 John Wiley & Sons, Ltd.

引用

页码：313 / 319

页数：7

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