Curcumin protects nigral dopaminergic neurons by iron-chelation in the 6-hydroxydopamine rat model of Parkinson's disease

被引：68
作者：
Du, Xi-Xun [1 ]
Xu, Hua-Min [1 ]
Jiang, Hong [1 ]
Song, Ning [1 ]
Wang, Jun [1 ]
Xie, Jun-Xia [1 ]
机构：
[1] Qingdao Univ, Coll Med, Dept Physiol, Shandong Prov Key Lab Pathogenesis & Prevent Neur, Qingdao 266071, Peoples R China
来源：
NEUROSCIENCE BULLETIN | 2012年 / 28卷 / 03期
基金：
中国国家自然科学基金;
关键词：
6-hydroxydopamine; curcumin; Parkinson's disease; dopaminergic neurons; iron; METAL TRANSPORTER 1; SUBSTANTIA-NIGRA; ANIMAL-MODELS; ANTIOXIDANT; CELLS; DEGENERATION; FLAVONOIDS; CADMIUM; BRAIN; MPTP;
D O I：
10.1007/s12264-012-1238-2
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Curcumin is a plant polyphenolic compound and a major component of spice turmeric (Curcuma longa). It has been reported to possess free radical-scavenging, iron-chelating, and anti-inflammatory properties in different tissues. Our previous study showed that curcumin protects MES23.5 dopaminergic cells from 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in vitro. The present study aimed to explore this neuroprotective effect in the 6-OHDAlesioned rat model of Parkinson's disease in vivo. Rats were given intragastric curcumin for 24 days. 6-OHDA lesioning was conducted on day 4 of curcumin treatment. Dopamine content was assessed by high-performance liquid chromatography with electrochemical detection, tyrosine hydroxylase (TH)-containing neurons by immunohistochemistry, and iron-containing cells by Perls' iron staining. The dopamine content in the striatum and the number of TH-immunoreactive neurons decreased after 6-OHDA treatment. Curcumin pretreatment reversed these changes. Further studies demonstrated that 6-OHDA treatment increased the number of iron-staining cells, which was dramatically decreased by curcumin pretreatment. The protective effects of curcumin against 6-OHDA may be attributable to the ironchelating activity of curcumin to suppress the iron-induced degeneration of nigral dopaminergic neurons.
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收藏
页码：253 / 258
页数：6
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