Overexpression of TLR2 and TLR4 susceptibility to serum deprivation-induced apoptosis in CHO cells

被引：20

作者：

Fan, W

Ha, TZ

Li, Y

Ozment-Skelton, T

Williams, DL

Kelley, J

Browder, IW

Li, CF ^{[1
]}

机构：

[1] E Tennessee State Univ, James H Quillen Coll Med, Dept Surg, Johnson City, TN 37614 USA

[2] E Tennessee State Univ, James H Quillen Coll Med, Dept Internal Med, Johnson City, TN 37614 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 337卷 / 03期

关键词：

toll-like receptors; serum deprivation; apoptosis; signaling transduction;

D O I：

10.1016/j.bbrc.2005.09.123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the effect of overexpression of TLR2 and TLR4 on apoptosis. TLR2 and TLR4 transfected CHO cells were subjected to serum deprivation for 0, 24, and 48 h. CHO cells served as control. The survival was 80.4% and 66.8% in CHO cells, 73.8% and 47.6% in TLR2/CHO, and 70.5% and 53.0% in TLR4/CHO, respectively. Flow cytometry examination suggested that apoptotic cells were 7.17% and 32.91% in control CHO cells, 29.0% and 64.6% in TLR2/CHO, and 41.4% and 64.6% in TLR4/CHO, respectively. The levels of FasL and caspase-8 activity in TLR2/CHO and TLR4/CHO cells were significantly higher than that of CHO cells. Transfection of dominant negative FADD into TLR2/CHO and TLR4/CHO cells significantly reduced apoptosis. Our results suggest that overexpression of TLR2 and TLR4 in CHO cells sensitizes the cells to serum deprivation-induced apoptosis and that the mechanisms are involved in the death receptor-mediated signaling pathway. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：840 / 848

页数：9

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