Tetramethylpyrazine Attenuates Renal Ischemia-Reperfusion Injury in Rats through Inhibiting Inflammation and Oxidative Stress and Enhancing Autophagy

In this study we evaluated the effect and mechanism of tetramethylpyrazine (TMP) on renal ischemia-reperfusion injury (RIRI) in rats. Forty-five SD rats were randomly divided into sham-operated (SO), RIRI and TMP+RIRI group, 15 rats in each group. The TMP+RIRI group was treated with 200 mg/kg TMP by intragastrical administration, once a day, for seven consecutive days. Then, the RIRI model was made in RIRI and TMP+RIRI groups. After 24 h from reperfusion, compared with RIRI group, in TMP+RIRI group the renal index and serum creatinine and blood urea nitrogen levels were decreased; the renal tissue tumor necrosis factor alpha, interleukin 1 beta and interleukin 6 levels were decreased, and the interleukin 10 level was increased; the renal tissue superoxide dismutase level was increased, and the malon-dialdehyde level was decreased; the renal tissue LC3-II/LC3-I ratio and Beclin-1/beta-actin ratio were increased. In conclusion, the TMP can attenuate the RIRI in rats through inhibiting the inflammation and oxidative stress and enhancing the autophagy in renal tissue.