INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of trans-Arachidonic Acids and Affects Eicosanoid Release during Storage

被引：5

作者：

Leitner, Gerda C. ^{[1
]}

Hagn, Gerhard ^{[2
]}

Niederstaetter, Laura ^{[2
]}

Bileck, Andrea ^{[2
,3
]}

Plessl-Walder, Kerstin ^{[4
]}

Horvath, Michaela ^{[1
]}

Kolovratova, Vera ^{[1
]}

Tanzmann, Andreas ^{[1
]}

Tolios, Alexander ^{[1
]}

Rabitsch, Werner ^{[5
]}

Wohlfarth, Philipp ^{[5
]}

Gerner, Christopher ^{[2
,3
]}

机构：

[1] Med Univ Vienna, Dept Blood Grp Serol & Transfus Med, A-1090 Vienna, Austria

[2] Univ Vienna, Fac Chem, Dept Analyt Chem, A-1090 Vienna, Austria

[3] Univ Vienna, Fac Chem, Joint Metabolome Facil, A-1090 Vienna, Austria

[4] Univ Appl Sci, Div Biomed Sci, FH Campus Wien, A-1100 Vienna, Austria

[5] Med Univ Vienna, Stem Cell Transplantat Unit, Internal Med 1, A-1090 Vienna, Austria

来源：

BIOMOLECULES | 2022年 / 12卷 / 09期

关键词：

eicosanoids; high-resolution mass spectrometry; liquid chromatography; pathogen reduction; platelet concentrates; platelets; trans-arachidonic acids; VERSUS-HOST-DISEASE; GRAFT;

D O I：

10.3390/biom12091258

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pathogen inactivation techniques for blood products have been implemented to optimize clinically safe blood components supply. The INTERCEPT system uses amotosalen together with ultraviolet light wavelength A (UVA) irradiation. Irradiation-induced inactivation of nucleic acids may actually be accompanied by modifications of chemically reactive polyunsaturated fatty acids known to be important mediators of platelet functions. Thus, here, we investigated eicosanoids and the related fatty acids released upon treatment and during storage of platelet concentrates for 7 days, complemented by the analysis of functional and metabolic consequences of these treatments. Metabolic and functional issues like glucose consumption, lactate formation, platelet aggregation, and clot firmness hardly differed between the two treatment groups. In contrast to gamma irradiation, here, we demonstrated that INTERCEPT treatment immediately caused new formation of trans-arachidonic acid isoforms, while 11-hydroxyeicosatetraenoic acid (11-HETE) and 15-HETE were increased and two hydroperoxyoctadecadienoic acid (HpODE) isoforms decreased. During further storage, these alterations remained stable, while the release of 12-lipoxygenase (12-LOX) products such as 12-HETE and 12-hydroxyeicosapentaenoic acid (12-HEPE) was further attenuated. In vitro synthesis of trans-arachidonic acid isoforms suggested that thiol radicals formed by UVA treatment may be responsible for the INTERCEPT-specific effects observed in platelet concentrates. It is reasonable to assume that UVA-induced molecules may have specific biological effects which need to be further investigated.

引用

页数：14