Efficient plant regeneration from cotyledonary node explants of Cucumis melo L.

被引:0
作者
Zhang, Huijun [1 ]
Peng, Gao [1 ]
Feishi, Luan [1 ]
机构
[1] NE Agr Univ, Hort Coll, Harbin 150030, Heilongjiang Pr, Peoples R China
来源
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 35期
基金
中国国家自然科学基金;
关键词
Cucumis Melo L; cotyledonary node; shoots regeneration; plant regeneration; AGROBACTERIUM-MEDIATED TRANSFORMATION; SOMATIC EMBRYOGENESIS; IN-VITRO; ORGANOGENESIS;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An efficient regeneration system was developed using cotyledonary node of two inbred line of melon, Cucumis melo 'CM-15' and 'CM-23'. The induction of shoots was achieved on Murashige and Skoog (MS) solid medium supplemented with different combinations of 6-benzylaminopurine (BA) and indole-3-acetic acid (IAA). The best shoot induction was observed when the explants were cultured on MS medium supplemented with BA (1.5 mg/L) and IAA (0.1 mg/L), and on MS medium supplemented with BA (1.5 mg/L) and IAA (0.5 mg/L) for 'CM-15' and 'CM-23', and inbred line CM-15 had the best regeneration rate. The shoot clumps were transferred to MS with BA (0.05 mg/L), resulting to the differentiation of most of the shoots initially into well developed shoots. Regenerated shoots were rooted on half-strength MS medium without plant growth regulators (PGRs). The rooted plants were established in soil with 100% success rate. This high frequency plant regeneration system provides improved technology to assist in genetic transformation of melon.
引用
收藏
页码:6757 / 6761
页数:5
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