Structural analysis of the jacalin-related lectin MornigaM from the black mulberry (Morus nigra) in complex with mannose

被引：30
作者：
Rabijns, A
Barre, A
Van Damme, EJM
Peumans, WJ
De Ranter, CJ
Rougé, P
机构：
[1] CNRS, UMR 5546, F-31326 Castanet Tolosan, France
[2] Katholieke Univ Leuven, Fac Pharmaceut Sci, Lab Analyt Chem & Med Physicochem, Louvain, Belgium
[3] State Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium
来源：
FEBS JOURNAL | 2005年 / 272卷 / 14期
关键词：
carbohydrate-binding site; jacalin-related lectin; mannose; Morus nigra; quaternary association;
D O I：
10.1111/j.1742-4658.2005.04801.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structures of MornigaM and the MornigaM-mannose complex have been determined at 1.8 angstrom and 2.0 angstrom resolution, respectively. Both structures adopt the typical beta-prism motif found in other jacalin-related lectins and their tetrameric assembly closely resembles that of jacalin. The carbohydrate-binding cavity of MornigaM readily binds mannose. No major structural rearrangements can be observed in MornigaM upon binding of mannose. These results allow corroboration of the structure-function relationships within the small group of Moraceae lectins.
引用
收藏
页码：3725 / 3732
页数：8
相关论文
共 40 条
  • [1] PURIFICATION AND PROPERTIES OF HEMAGGLUTININ FROM MACLURA-POMIFERA SEEDS
    BAUSCH, JN
    PORETZ, RD
    [J]. BIOCHEMISTRY, 1977, 16 (26) : 5790 - 5794
  • [2] PROTEIN DATA BANK - COMPUTER-BASED ARCHIVAL FILE FOR MACROMOLECULAR STRUCTURES
    BERNSTEIN, FC
    KOETZLE, TF
    WILLIAMS, GJB
    MEYER, EF
    BRICE, MD
    RODGERS, JR
    KENNARD, O
    SHIMANOUCHI, T
    TASUMI, M
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1977, 112 (03) : 535 - 542
  • [3] Helianthus tuberosus lectin reveals a widespread scaffold for mannose-binding lectins
    Bourne, Y
    Zamboni, V
    Barre, A
    Peumans, WJ
    Van Damme, EJM
    Rougé, P
    [J]. STRUCTURE, 1999, 7 (12) : 1473 - 1482
  • [4] Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose
    Bourne, Y
    Astoul, CH
    Zamboni, V
    Peumans, WJ
    Menu-Bouaouiche, L
    Van Damme, EJM
    Barre, A
    Rougé, P
    [J]. BIOCHEMICAL JOURNAL, 2002, 364 (01) : 173 - 180
  • [5] Brunger AT, 1998, ACTA CRYSTALLOGR D, V54, P905, DOI 10.1107/s0907444998003254
  • [6] BRUNGER AT, 1992, X PLOR VERSION 3 1 S
  • [7] An extensively modified version of MolScript that includes greatly enhanced coloring capabilities
    Esnouf, RM
    [J]. JOURNAL OF MOLECULAR GRAPHICS & MODELLING, 1997, 15 (02) : 132 - +
  • [8] Two jasmonate-inducible myrosinase-binding proteins from Brassica napus L. seedlings with homology to jacalin
    Geshi, N
    Brandt, A
    [J]. PLANTA, 1998, 204 (03) : 295 - 304
  • [9] CALCULATION OF ELECTROSTATIC POTENTIALS IN AN ENZYME ACTIVE-SITE
    GILSON, MK
    HONIG, BH
    [J]. NATURE, 1987, 330 (6143) : 84 - 86
  • [10] ESPript:: analysis of multiple sequence alignments in PostScript
    Gouet, P
    Courcelle, E
    Stuart, DI
    Métoz, F
    [J]. BIOINFORMATICS, 1999, 15 (04) : 305 - 308
1234