Synergistic Effect of Ischemic Preconditioning, Postcoditioning and Xanthine Oxidase Inhibition on Cardiac Tissue apoptosis of Hepatic Ischemic-Reperfused Male Rats

Accumulating evidences have recently documented that hepatic ischemic mechanical preconditioning, postconditioning or ischemic pharmacological preconditioning had protective effects on the liver, which were associated with a reduction in oxidative stress, inflammation and endogenous antioxidant preservation. However, assessment of cardioprotective effects of remote hepatic ischemic preconditioning, postconditioning or pharmacological preconditioning is unclear and needs further investigations. The aim of this study was to investigate the remote effect of hepatic ischemia/reperfusion (IR) on cardiac tissue. And to investigate whether hepatic ischemic preconditioning (IPC), postconditioning (IPO) and/or pharmacological preconditioning by xanthine oxidase inhibitor (allopurinol) (Allo) may extend a beneficial synergistic effect to protect the cardiac tissue. Forty male Albino rats were divided into 5 experimental groups: group I: sham-operated controls, group II: Hepatic I/R, group III: IPC+ I/R+ IPO, group IV: Allo + I/R, group V: Allo+IPC+I/R+IPO. Serum interleukin-6, cardiac malondialdehyde (MDA), reduced form of glutathione (GSH), Bax and Bcl-2 mRNA expressions were measured at the end of experiment. Results revealed that IPC, IPO and/or Allo treatment significantly reduced the levels of IL-6, MDA, Bax mRNA and Bax/Bcl-2 ratio and significantly increased the levels of GSH and Bcl-2 mRNA. In conclusion: IPC, IPO and Allo treatment may act synergistically to protect cardiac tissue against oxidative stress and mitochondrial injury during hepatic I/R. [Hanan A. Mubarak Synergistic Effect of Ischemic Preconditioning, Postcoditioning and Xanthine Oxidase Inhibition on Cardiac Tissue apoptosis of Hepatic Ischemic-Reperfused Male Rats] Life Science Journal,. 2011; 8(4): 253-262] (ISSN: 1097-8135). http://www.lifesciencesite.com.