Quercetin promotes MC3T3-E1 cell growth via PI3K/Akt signaling pathway

被引：5
作者：
Liang, Haidong [1 ]
Yu, Fang [1 ]
机构：
[1] Dalian Med Univ, Dalian, Peoples R China
来源：
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | 2018年 / 17卷 / 12期
关键词：
Quercetin; MC3T3-E1; cells; AKT phosphorylation; Osteopontin; Osteoprotegerin; Osteocalcin; RunX-2; BONE; DIFFERENTIATION; FLAVONOIDS; INHIBITION; FRACTURE;
D O I：
10.4314/tjpr.v17i12.8
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Purpose: To investigate the effect of quercetin on mouse osteoblast MC3T3-E1 cell growth and the molecular mechanisms involved. Methods: The effect of quercetin on MC3T3-E1 cells growth, cell cycle distribution, bone-related genes and PI3K/Akt signaling pathway were determined in vitro using MTT assay, flow cytometry, qPCR and western blot, respectively. Results: Qercetin promoted MC3T3-E1 cell growth, as revealed from MTT assay. Flow cytometry showed that quercetin administration resulted in accumulation of cells in the S phase. In addition, quercetin up-regulated mRNA expression levels of osteopontin, ALP, osteoprotegerin, osteocalcin and RunX-2, and increased phosphorylation of AKT. Conclusion: Quercetin enhances the growth of MC3T3-E1 cells via a mechanism involving accumulation of cells in S phase, increasing mRNA expression levels of bone-associated genes, and increasing the phosphorylation of AKT.
引用
收藏
页码：2371 / 2374
页数：4
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