A concise method for quantitative analysis of interactions between lipids and membrane proteins

被引:13
作者
Inada, Masataka [1 ]
Kinoshita, Masanao [1 ]
Sumino, Ayumi [2 ,3 ,4 ]
Oiki, Shigetoshi [2 ]
Matsumori, Nobuaki [1 ]
机构
[1] Kyushu Univ, Fac Sci, Dept Chem, Nishi Ku, 744 Motooka, Fukuoka, Fukuoka 8190395, Japan
[2] Univ Fukui, Fac Med Sci, Dept Mol Physiol & Biophys, Fukui 9101193, Japan
[3] Kanazawa Univ, Inst Frontier Sci Initiat, High Speed AFM Biol Applicat Unit, Kanazawa, Ishikawa 9201192, Japan
[4] Kanazawa Univ, BioAFM Frontier Res Ctr, Kanazawa, Ishikawa 9201192, Japan
关键词
Lipid; Membrane protein; Quantitative interaction analysis; Surface plasmon resonance; Self-assembled monolayer; Bacteriorhodopsin; SURFACE-PLASMON RESONANCE; CRYSTAL-STRUCTURE; BIOLOGICAL-MEMBRANES; PURPLE MEMBRANE; K+ CHANNEL; BACTERIORHODOPSIN; BINDING; MODELS; ETHER;
D O I
10.1016/j.aca.2019.01.042
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Although interactions between lipids and membrane proteins (MPs) have been considered crucially important for understanding the functions of lipids, lack of useful and convincing experimental methods has hampered the analysis of the interactions. Here, we developed a surface plasmon resonance (SPR)-based concise method for quantitative analysis of lipid-MP interactions, coating the sensor chip surface with self-assembled monolayer (SAM) with C-6-chain. To develop this method, we used bacteriorhodopsin (bR) as an MP, and examined its interaction with various types of lipids. The merits of using C-6-SAM-modified sensor chip are as follows: (1) alkyl-chains of SAM confer a better immobilization of MPs because of the efficient preconcentration due to hydrophobic contacts; (2) SAM provides immobilized MPs with a partial membranous environment, which is important for the stabilization of MPs; and (3) a thinner C-6-SAM layer (1 nm) compared with MP size forces the MP to bulge outward from the SAM surface, allowing extraneously injected lipids to be accessible to the hydrophobic transmembrane regions. Actually, the amount of bR immobilized on C-6 -SAM is 10 times higher than that on a hydrophilic CM5 sensor chip, and AFM observations confirmed that bR molecules are exposed on the SAM surface. Of the lipids tested, S-TGA-1, a halobacterium-derived glycolipid, had the highest specificity to bR with a nanomolar dissociation constant. This is consistent with the reported co-crystal structure that indicates the formation of several intermolecular hydrogen bonds. Therefore, we not only reproduced the specific lipid-bR recognition, but also succeeded in its quantitative evaluation, demonstrating the validity and utility of this method. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:103 / 112
页数:10
相关论文
共 57 条
  • [1] X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors
    Althoff, Thorsten
    Hibbs, Ryan E.
    Banerjee, Surajit
    Gouaux, Eric
    [J]. NATURE, 2014, 512 (7514) : 333 - +
  • [2] Novel ether lipid cardiolipins in archaeal membranes of extreme haloalkaliphiles
    Angelini, Roberto
    Corral, Paulina
    Lopalco, Patrizia
    Ventosa, Antonio
    Corcelli, Angela
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2012, 1818 (05): : 1365 - 1373
  • [3] MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine
    Angelini, Roberto
    Babudri, Francesco
    Lobasso, Simona
    Corcelli, Angela
    [J]. JOURNAL OF LIPID RESEARCH, 2010, 51 (09) : 2818 - 2825
  • [4] Isothermal Microcalorimetry to Investigate Non Specific Interactions in Biophysical Chemistry
    Ball, Vincent
    Maechling, Clarisse
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2009, 10 (08) : 3283 - 3315
  • [5] Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers
    Bayburt, TH
    Sligar, SG
    [J]. PROTEIN SCIENCE, 2003, 12 (11) : 2476 - 2481
  • [6] BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
  • [7] Surface plasmon resonance analysis of ricin binding to plasma membranes isolated from NIH 3T3 cells
    Blome, Matthew C.
    Petro, Kimberly A.
    Schengrund, Cara-Lynne
    [J]. ANALYTICAL BIOCHEMISTRY, 2010, 396 (02) : 212 - 216
  • [8] The influence of membrane lateral pressures on simple geometric models of protein conformational equilibria
    Cantor, RS
    [J]. CHEMISTRY AND PHYSICS OF LIPIDS, 1999, 101 (01) : 45 - 56
  • [9] Effects of Tween 20® and Tween 80® on the stability of albutropin during agitation
    Chou, DK
    Krishnamurthy, R
    Randolph, TW
    Carpenter, JF
    Manning, MC
    [J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 2005, 94 (06) : 1368 - 1381
  • [10] Specificity of Intramembrane Protein-Lipid Interactions
    Contreras, Francesc-Xabier
    Ernst, Andreas Max
    Wieland, Felix
    Bruegger, Britta
    [J]. COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2011, 3 (06): : 1 - 18