Bacillus kiskunsagensis sp nov., a novel alkaliphilic and moderately halophilic bacterium isolated from soda soil

An alkaliphilic and moderately halophilic strain characterized by optimal growth at pH 9.0-10.0 and 7% (w/v) NaCl, and designated B16-24(T), was isolated from the rhizosphere soil of the bayonet grass Bolboschoenus maritimus at a soda pond in the Kiskunsag National Park, Hungary. Cells of the strain were Gram-staining-positive, non-motile, straight rods, and formed central, ellipsoidal endospores with slightly swollen sporangia. The isolate was facultative anaerobic, catalase positive, oxidase negative, and contained a peptidoglycan of type A1 gamma based on meso-diaminopimelic acid. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone, and anteiso-C-15:0 the major cellular fatty acid. The DNA G+C content of strain B16-24(T) was 36.6 mol%. The 16S rRNA gene-based phylogenetic analysis revealed that the novel isolate had the greatest similarities to the type strains of Bacillus okhensis Kh10-101(T) (97.8 %), B. akibai 1139(T) (97.4 %), B. alkalisediminis K1-25(T) (97.3 %) and B. wakoensis N-1(T) (97.1 %). The DNA-DNA relatedness of strain B16-24(T) and the closely related Bacillus species ranged between 24 +/- 6% and 35 +/- 3 %. The distinctive phenotypic and genetic results of this study confirmed that strain B16-24(T) represents a novel species within the genus Bacillus, for which the name Bacillus kiskunsagensis sp. nov. is proposed. The type strain is B16-24(T) (=DSM 29791(T)=NCAIM B. 02610(T)).