Immunocytochemical localization of prostaglandin EP3 receptor in the rat hypothalamus

被引：68
|
作者：
Nakamura, K
Kaneko, T
Yamashita, Y
Hasegawa, H
Katoh, H
Ichikawa, A
Negishi, M [1 ]
机构：
[1] Kyoto Univ, Fac Pharmaceut Sci, Dept Mol Neurobiol, Sakyo Ku, Kyoto 6068501, Japan
[2] Kyoto Univ, Grad Sch Med, Dept Morphol Brain Sci, Sakyo Ku, Kyoto 6068501, Japan
[3] Kyoto Univ, Fac Pharmaceut Sci, Dept Physiol Chem, Sakyo Ku, Kyoto 6068501, Japan
来源：
NEUROSCIENCE LETTERS | 1999年 / 260卷 / 02期
关键词：
prostaglandin E-2; EP3; receptor; antibody; immunoblotting; rat; hypothalamus; fever mechanism; immunocytochemistry;
D O I：
10.1016/S0304-3940(98)00962-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
A rabbit antibody against an N-terminal portion of rat prostaglandin EP3 receptor (EP3R) was produced to examine the distribution of EP3R in the rat hypothalamus. The antibody specifically recognized EP3R proteins in rat brain extract, in membrane fractions of rat kidney, and in membrane fractions of EP3R-expressing culture cells. Intense EP3R-like immunoreactivity was observed in the median preoptic nucleus, medial preoptic area, parastrial nucleus, compact part of the dorsomedial hypothalamic nucleus, and dorsal part of the premammillary nucleus. These results suggest that prostaglandin Ep mediates various actions in the hypothalamus, such as fever induction in the preoptic area, through EP3R. (C) 1999 Elsevier Science ireland Ltd. All rights reserved.
引用
收藏
页码：117 / 120
页数：4
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